Evaluation of antimycobacterial activity of Curcuma xanthorrhiza ethanolic extract against Mycobacterium tuberculosis H37Rv in vitro

Vet World Vol.11 March-2018 Article-18

Research Article

Veterinary World, 11(3): 368-372

https://doi.org/10.14202/vetworld.2018.368-372

Ngadino¹, Setiawan¹, Koerniasari¹, Ernawati², and S. A. Sudjarwo³

1. Study Program of Environmental Health, Polytechnic of Health, Surabaya, Indonesia.
2. Department of Pharmacology, Faculty of Medicine, Wijaya Kusuma University, Surabaya, Indonesia.
3. Department of Pharmacology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia.

Background and Aim: The aim of this study was to evaluate the antimycobacterial activity of the Curcuma xanthorrhiza ethanolic extract in vitro.

Materials and Methods: Ethanolic extract of C. xanthorrhiza was set by maceration method. The broth microdilution and disc diffusion method were used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC), respectively, of C. xanthorrhiza ethanol extract on strain Mycobacterium tuberculosis H37Rv.

Results: C. xanthorrhiza ethanol extract was found to have the antimycobacterial effects with a MIC value of 1600 μg/ml while MBC value of 3200 μg/ml for M. tuberculosis H37Rv.

Conclusion: From these findings , it can be concluded that C. xanthorrhiza ethanol extract have an antibacterial activity against Mycobacterium tuberculosis H37Rv in vitro and its potency elevated by increasing the C. xanthorrhiza ethanol extract concentration. Keywords: antimycobacterial, Curcuma xanthorrhiza, minimal bactericidal concentration, minimal inhibitory concentration.

Keywords: antimycobacterial, Curcuma xanthorrhiza, minimal bactericidal concentration, minimal inhibitory concentration.

How to cite this article: Ngadino, Setiawan, Koerniasari, Ernawati, Sudjarwo SA (2018) Evaluation of antimycobacterial activity of Curcuma xanthorrhiza ethanolic extract against Mycobacterium tuberculosis H37Rv in vitro, Veterinary World, 11(3): 368-372.

Received: 09-12-2017 Accepted: 20-02-2018 Published online: 27-03-2018

Corresponding author: S. A. Sudjarwo E-mail: ags158@yahoo.com

DOI: 10.14202/vetworld.2018.368-372

Copyright: Ngadino, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.