Vet World Vol.11 November-2018 Article-17
Research Article
Veterinary World, 11(11): 1630-1636
https://doi.org/10.14202/vetworld.2018.1630-1636
The first evidence of a new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and unvaccinated broiler flocks in Algeria
2. Department of natural sciences and life, Faculty of Sciences and Technology, University Yahia Fares of Medea, Algeria.
3. Microbes, Evolution, Phylogeny and Infection, UMR Aix-Marseille University, IRD, APHM, IHU Mediterranee Infection, Marseille, France.
Background and Aim: Avian infectious bronchitis virus (IBV) frequently infects broilers and is responsible for severe economic losses to the poultry industry worldwide. It has also been associated with kidney damage in the broiler flocks. The aim of the present study is to determine the presence of IBV and its possible involvement in kidney damage of broiler chicks.
Materials and Methods: 14 clinically diseased broiler flocks from Western and Central Algeria were sampled and analyzed by hemagglutination inhibition (HI) test and reverse transcriptase-polymerase chain reaction (RT-PCR) followed by phylogenic analysis.
Results: The QX (100%) and 4/91 (60%) IBV serotypes were the most prevalent in the kidney damaged broilers regardless of vaccination status. The molecular detection of avian IBV by RT-PCR identified six samples as positive, of which only two isolates were typable by sequencing. We identified a novel IBDZ13a genotype which showed 93% sequence homology to the partial-S1 gene sequence of the IB 4/91 commercial vaccine strain. Sequencing analysis characterized this virus as a novel and divergent IB 4/91 field virus with eight amino acid substitutions that might have resulted in altered immunogenicity.
Conclusion: The isolation of a new IBV strain (IBDZ13a) from vaccinated broiler flocks may explain the failure of the vaccination programs against IBV field strains. Combination of the HI test and RT-PCR indicated that the nephropathogenic IB outbreaks in broilers are related to this novel strain. Keywords: avian infectious bronchitis virus, broilers, kidney damages, Algeria, hemagglutination inhibition test, reverse transcriptase-polymerase chain reaction, IBDZ13a.
Keywords: avian infectious bronchitis virus, broilers, kidney damages, Algeria, hemagglutination inhibition test, reverse transcriptase-polymerase chain reaction, IBDZ13a.
How to cite this article: Lounas A, Oumouna-Benachour K, Medkour H, Oumouna M (2018) The first evidence of a new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and unvaccinated broiler flocks in Algeria, Veterinary World, 11(11): 1630-1636.
Received: 16-07-2018 Accepted: 12-10-2018 Published online: 29-11-2018
Corresponding author: A. Lounas E-mail: azizovet@yahoo.fr
DOI: 10.14202/vetworld.2018.1630-1636
Copyright: Lounas, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.