Vet World   Vol.11   September-2018  Article-12

Evaluation of specific and non-specific immune response of four vaccines for caseous lymphadenitis in sheep challenged

Background and Aim: The present study was directed to perform a comparative innate and acquired immune response assessment of different four vaccine formulas to evoke protection against induced (CLA) challenge in sheep.

Materials and Methods: Negative ELISA (free of CLA) 15 local breed male (Balady) sheep were divided into five groups, each has received a different vaccine while the control has received saline buffer. The first vaccine composed of toxoid PLD alone the second composed of toxoid PLD with bacterin (formalinkilled bacteria), the third vaccine composed of toxoid PLD plus covaccine 8, while the fourth one composed of toxoid PLD plus locally produced polyvalent clostridial vaccine. The specific immune response was evaluated through lymphocyte proliferation assay using ELISA BrdU kit, while the non-specific response was estimated by superoxide anion production and lysozyme activity assays.

Results: The study revealed that PLD toxoid could evoke the highest specific immune response, showing a stimulation index (9.12%). On the other hand, combined toxoid PLD with bacterin followed by PLD toxoid showed a significant increase in the non-specific innate immune response.

Conclusion: The present study indicated that the toxoid PLD alone vaccine was most efficient and provided innate and acquired immune response in animals against CLA. Keywords: Corynebacterium pseudotuberculosis, immune response, lymphocyte, phospholipase D, vaccine.

Keywords: Corynebacterium pseudotuberculosis, immune response, lymphocyte, phospholipase D, vaccine.

How to cite this article: Syame SM, Abuelnaga ASM, Ibrahim ES, Hakim AS (2018) Evaluation of specific and non-specific immune response of four vaccines for caseous lymphadenitis in sheep challenged, Veterinary World, 11(9): 1272-1276.

Received: 17-05-2018  Accepted: 31-07-2018     Published online: 17-09-2018

Corresponding author: Sohier M. Syame   E-mail: sohiersyame@yahoo.com

DOI: 10.14202/vetworld.2018.1272-1276

Copyright: Syame, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.