Cloning and sequencing gB, gD, and gM genes to perform the genetic variability of bovine herpesvirus-1 from Indonesia

Vet World Vol.11 September-2018 Article-9

Research Article

Veterinary World, 11(9): 1255-1261

https://doi.org/10.14202/vetworld.2018.1255-1261

Dewi Noor Hidayati^1,2, Tri Untari³, Michael Haryadi Wibowo³, Koichi Akiyama⁴, and Widya Asmara³

1. Doctoral Program, Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna No. 2, Caturtunggal, Depok, Karangmalang, Sleman, Daerah Istimewa Yogyakarta 55281, Indonesia.
2. PUSVETMA (Pusat Veteriner Farma), The Ministry of Agriculture of The Republic of Indonesia, Jl. Frontage Ahmad Yani Siwalankerto No. 68-70, Ketintang, Gayungan, Surabaya, Jawa Timur 60231, Indonesia.
3. Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna No. 2, Caturtunggal, Depok, Karangmalang, Sleman, Daerah Istimewa Yogyakarta 55281, Indonesia.
4. Advanced Research Support Centre (ADRES), Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan.

Background and Aim: Previous research has shown that bovine herpesvirus-1 (BHV-1) in Indonesia was closely related to subtype-1 based on glycoprotein D genes. This study aimed to analyze the genetic variability of the BHV-1 isolated from the recent case in Indonesia not only based on gD but also other genes such as gB and gM and to study the homology and similarity of the sample to other BHV-1 isolated in other countries or regions.

Materials and Methods: Samples were drawn from the tracheal organ in recent field case and prepared for DNA extraction. The gB, gD, and gM were amplified using nested polymerase chain reaction (nPCR) with our specifically designed primer pair and based on the specified bands of 350 bp gB, 325 bp gD, and 734 bp gM confirmed as BHV-1. The PCR product was ligated into pGEM-T and transformed into competent Escherichia coli. The purified plasmid was subsequently sequenced.

Results: The virus sample isolated from the recent field case of infectious bovine rhinotracheitis (IBR) from Indonesia showed variability based on the gB, gD, and gM sequences. However, all of the genes had high similarity (98-100%) to BHV-1.2.

Conclusion: The recent field case of IBR in Indonesia was similar to BHV-1.2. Keywords: bovine herpesvirus-1.1, bovine herpesvirus-1.2, glycoprotein B, glycoprotein D, glycoprotein M.

Keywords: bovine herpesvirus-1.1, bovine herpesvirus-1.2, glycoprotein B, glycoprotein D, glycoprotein M.

How to cite this article: Hidayati DN, Untari T, Wibowo MH, Akiyama K, Asmara W (2018) Cloning and sequencing gB, gD, and gM genes to perform the genetic variability of bovine herpesvirus-1 from Indonesia, Veterinary World, 11(9): 1255-1261.

Received: 09-03-2018 Accepted: 31-07-2018 Published online: 14-09-2018

Corresponding author: Widya Asmara E-mail: wied_as@ugm.ac.id

DOI: 10.14202/vetworld.2018.1255-1261

Copyright: Hidayati, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.