Effects of four extenders on the quality of frozen semen in Arabian stallions

Vet World Vol.12 January-2019 Article-4

Research Article

Veterinary World, 12(1): 34-40

https://doi.org/10.14202/vetworld.2019.34-40

Mohaammed Saad Alamaary^1,2, Abd Wahid Haron¹, Mohamed Ali³, Mark Wen Han Hiew¹, Lawan Adamu^1,4, and Innocent Damudu Peter^1,4

1. Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
2. King Abdulaziz Arabian Horses Center, Ministry of Agriculture, Riyadh, Saudi Arabia.
3. Department of Animal Production and Breeding, Faculty of Agriculture and Veterinary Medicine, AL Qassim University, AL Qassim State, Saudi Arabia.
4. Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Nigeria.

Background and Aim: Different types of extenders have a variety of components which show the tolerance effect on sperm protection during freezing procedures. In the present study, we have examined the impact of the extenders HF-20 and Tris, which were locally manufactured, and they are competing with commercial extenders INRA Freeze® (IMV Technologies, France) and EquiPlus Freeze® (Minitube, Germany) on the quality of horses frozen semen.

Materials and Methods: A total of 15 ejaculates from three healthy stallions were collected and cryopreserved in the same environment. Each semen sample collected was divided into four equal parts and processed. All samples were analyzed before and after freezing for motility, viability, plasma membrane integrity, and morphology. Furthermore, twenty mares were inseminated using post-thawed semen.

Results: There were no differences observed among all extenders in all the parameters before freezing. Sperm cryopreserved using HF-20 showed better motility, viability, and plasma membrane integrity than Tris extender. The Tris extender showed the most inferior quality of post-thawed semen between all the extenders. HF-20, INRA Freeze®, and EquiPlus Freeze® extenders revealed the same capacity of semen preservation in vitro and in vivo.

Conclusion: HF-20 extender has the same quality as INRA Freeze® and EquiPlus Freeze® that can be considered as one of the best extenders for the semen cryopreservation in horses. In contrast, Tris extender needs some degree of improvement. Keywords: Arabian stallion, frozen semen, semen, extenders.

Keywords: Arabian stallion, frozen semen, semen, extenders.

How to cite this article: Alamaary MS, Wahid H, Ali M, Hiew MWH, Adamu L, Peter ID (2019) Effects of four extenders on the quality of frozen semen in Arabian stallions, Veterinary World, 12(1): 34-40.

Received: 10-07-2018 Accepted: 14-11-2018 Published online: 07-01-2019

Corresponding author: Abd Wahid Haron E-mail: wahidh@upm.edu.my

DOI: 10.14202/vetworld.2019.34-40

Copyright: Alamaary, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.