Tadpole serum activity (Rana catesbeiana) in caspase-3 as a marker of the role of apoptosis and total cytotoxic T lymphocytes in albino rats' epithelial cells induced by neoplasia

Vet World Vol.12 January-2019 Article-8

Research Article

Veterinary World, 12(1): 63-67

https://doi.org/10.14202/vetworld.2019.63-67

M. T. E. Purnama¹, I. H. Rahmaningtyas¹, A. R. Pratama¹, Z. Prastika¹, A. M. Kartikasari², and N. P. D. Cahyo²

1. Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Mulyorejo Campus C Surabaya, East Java 60115, Indonesia.
2. Department of Veterinary Pathology, Faculty of Veterinary Medicine, Universitas Airlangga, Mulyorejo Campus C Surabaya, East Java 60115, Indonesia.

Background and Aim: This study was conducted to examine the tadpole's serum activity (Rana catesbeiana) in caspase-3 as a marker of the role of apoptosis and total cytotoxic T lymphocyte (CTL) in albino rats' epithelial cells induced by neoplasia. Tadpole serumcontains thyroxine hormone that may cause the metamorphosis process and control cell proliferation.

Materials and Methods: Male rats were induced by 7,12-dimethylbenz (α)anthracene (DMBA) 20 mg/rats twice every week over 5 weeks to stimulate skin neoplasia. Tadpole serum injected intracutaneously after neoplasia is known. The negative control group (C−) was not exposed to DMBA and tadpole serum, while the positive control group (C+) was exposed to DMBA. Treatment groups (T1, T2, and T3) were exposed DMBA and tadpole serum 100%, 75%, and 25%/rat/ day, respectively. Samples of skin organ were be made preparations immunohistochemistry interacted with caspase-3 and CTL antibody as the marker.

Results: Based on the result, immunohistochemistry from skin neoplasia and given therapy of tadpole serum show that Treatment 1 was the highest caspase-3 and CTL expression. The result of caspase-3 expression in C−, C+, T1, T2, and T3 was 0.00c±0.000, 0.70bc±0.141, 2.00a±0.283, 1.10b±0.424, and 1.15b±0.495, respectively. The result of CTL expression in C−, C+, T1, T2, and T3 was 0.10d±0.200, 1.00c±0.230, 2.10a±0.529, 1.70ab±0.258, and 1.35bc±0.443, respectively.

Conclusion: It can be concluded from the study that tadpole serum (R. catesbeiana) 100% concentration can increase caspase-3 and total CTL in albino rats' epithelial cells induced by neoplasia. Keywords: caspase-3, cytotoxic T lymphocyte, Rana catesbeiana, serum, tadpoles.

Keywords: caspase-3, cytotoxic T lymphocyte, Rana catesbeiana, serum, tadpoles.

How to cite this article: Purnama MTE, Rahmaningtyas IH, Pratama AR, Prastika Z, Kartikasari AM, Cahyo NPD (2019) Tadpole serum activity (Rana catesbeiana) in caspase-3 as a marker of the role of apoptosis and total cytotoxic T lymphocytes in Albino rats epithelial cells induced by neoplasia, Veterinary World, 12(1): 63-67.

Received: 10-08-2018 Accepted: 26-11-2018 Published online: 10-01-2019

Corresponding author: M. T. E. Purnama E-mail: thohawi@fkh.unair.ac.id

DOI: 10.14202/vetworld.2019.63-67

Copyright: Purnama MTE, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.