Vet World   Vol.12   July-2019  Article-28

A diluent containing coconut water, fructose, and chicken egg yolk increases rooster sperm quality at 5°C

Background and Aim: The present study was conducted to evaluate the quality of rooster sperm at 5°C after treatment with a diluent containing coconut water, fructose, and chicken egg yolk and stored the semen sample at 5°C.

Materials and Methods: Ten semen samples from 10 healthy roosters were subjected to four different treatments. For the treatments, 0.2 ml fresh semen with a sperm concentration of 5.2X109 cell/ml was mixed with T0 (no diluent), T1 (0.34 ml coconut water and 6 μl fructose), T2 (0.274 ml coconut water, 0.12 ml egg yolk, and 6 μl fructose), and T3 (0.34 ml egg yolk and 6 μl fructose) solutions. Each treated solution was stored at 5°C and evaluated both macroscopically and microscopically. Macroscopically, semen volume, pH, and sperm concentration were evaluated. The microscopic sperm characteristics examined included total motility (i.e., rapid, medium, or slow), progressive and non-progressive motility, viability, and spermatozoa abnormalities noted at different storage times. The results showed that spermatozoa motility was under 40%.

Results: The results indicated that sperm viability significantly affected (p<0.05). The highest mean value of sperm viability on day 7 of storage was found after treatment with the T2 solution (46.100±0.5677%). Similarly, spermatozoa abnormalities were significantly lower after treatment with the T2 solution (6.680±1.702%).

Conclusion: The addition of a diluent containing coconut water, egg yolk, and fructose helped in the better preservation spermatozoa motility, as well as viability for up to 7 days when the semen samples were stored at 5°C. Keywords: chicken, coconut water, diluent, egg yolk, sperm quality.

Keywords: chicken, coconut water, diluent, egg yolk, sperm quality.

How to cite this article: Rochmi SE, Sofyan MS (2019) A diluent containing coconut water, fructose, and chicken egg yolk increases rooster sperm quality at 5°C, Veterinary World, 12(7): 1116-1120.

Received: 25-01-2019  Accepted: 14-06-2019     Published online: 25-07-2019

Corresponding author: Siti Eliana Rochmi   E-mail: eliana.rochmi@vokasi.unair.ac.id

DOI: 10.14202/vetworld.2019.1116-1120

Copyright: Rochmi and Sofyan, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.