Detection of virulence and multidrug resistance operons in Pseudomonas aeruginosa isolated from Egyptian Baladi sheep and goat

Vet World Vol.12 October-2019 Article-2

Research Article

Veterinary World, 12(10): 1524-1528

https://doi.org/10.14202/vetworld.2019.1524-1528

A. N. Dapgh¹, A. S. Hakim², H. A. Abouelhag², A. M. Abdou², and E. A. Elgabry²

1. Department of Bacteriology, Animal Health Research Institute, Dokki, Giza, Egypt.
2. Department of Microbiology and Immunology, National Research Centre, 33 Bohouth Street, 12622 Dokki, Cairo, Egypt.

Background and Aim: The current study was carried out to illustrate the prevalence of P. aeruginosa in small ruminants and existence of some virulence operons as well as its antimicrobial resistance.

Materials and Methods: A total of 155 samples from sheep and 105 samples from goats (mouth abscesses, fecal swabs, nasal, tracheal swabs, and lung tissue) were collected for bacteriological study, existence of some virulence expression operons with the study of their sensitivity to the antimicrobials using disc diffusion and presence of mexR operon which is responsible for multidrug resistance (MDR).

Results: The bacteriological examination revealed that P. aeruginosa was isolated from nine out of 155 samples from sheep (5.8%) and four isolates out of 105 samples from goat (3.8%). It is found that 12 (92.3%), 10 (76.9 %), and 8 (61.5%) of P. aeruginosa isolates harbored hemolysin phospholipase gene (pclH), gene (exoS), and enterotoxin gene (toxA), respectively. The results of antibiotic sensitivity test showed that all tested isolates were resistant to ampicillin, bacitracin, erythromycin, streptomycin, tetracycline, trimethoprim-sulfamethoxazole, and tobramycin but sensitive to ciprofloxacin and norfloxacin. The MDR (mexR) operon was existed in all isolates.

Conclusion: There is a growing risk for isolation of virulent MDR P. aeruginosa from sheep and goat illness cases, and this should be regarded in the efficient control programs. Keywords: drug resistance, goat, Pseudomonas aeruginosa, sheep, virulence.

Keywords: drug resistance, goat, Pseudomonas aeruginosa, sheep, virulence.

How to cite this article: Dapgh AN, Hakim AS, Abouelhag HA, Abdou AM, Elgabry EA (2019) Detection of virulence and multidrug resistance operons in Pseudomonas aeruginosa isolated from Egyptian Baladi sheep and goat, Veterinary World, 12(10): 1524-1528.

Received: 07-05-2019 Accepted: 26-08-2019 Published online: 04-10-2019

Corresponding author: A. S. Hakim E-mail: migris410@yahoo.com

DOI: 10.14202/vetworld.2019.1524-1528

Copyright: Dapgh, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.