Vet World Vol.13 April-2020 Article-16
Research Article
Veterinary World, 13(4): 726-730
https://doi.org/10.14202/vetworld.2020.726-730
In vitro assessment of time-dependent changes in red cell cytoplasmic antioxidants of donkey blood preserved in citrate phosphate dextrose adenine 1 anticoagulant
2. Department of Veterinary Physiology and Pharmacology, Faculty of Veterinary Medicine, University of Nigeria, Enugu State, Nigeria.
3. Veterinary Teaching Hospital, Faculty of Veterinary Medicine, University of Nigeria, Enugu State, Nigeria.
Background and Aim: Stored blood is continuously exposed to oxidative stress, which affects its antioxidant protective system. Erythrocytes are naturally armed with antioxidant protective capacity. Blood antioxidant system functions to protect the blood cells against oxidative damage by free radicals. However, during storage, blood is continuously exposed to oxidative stress, which affects its antioxidant system. The aim of this work was to investigate alteration in malondialdehyde (MDA) levels, reduced glutathione (glutathione reductase [GSH-Rd]), catalase (CAT), and superoxide dismutase (SOD) activities in stored donkey blood.
Materials and Methods: Blood (250 ml) was drawn from four clinically healthy donkeys into citrate phosphate dextrose adenine 1 blood bags and preserved at 4°C. MDA, GSH-Rd, CAT, and SOD activities were assayed by colorimetric methods, over a period of 42 days.
Results: The result showed that SOD enzyme activities significantly (p<0.05) increased by day 7 post-storage (PS) while MDA levels significantly (p<0.05) increased by day 21 PS. However, activities of GSH-Rd and CAT enzymes decreased (p<0.05) by day 21 PS. Pearson's product-moment correlation showed a negative correlation between the levels of MDA and enzymatic antioxidant markers (CAT and GSH-Rd).
Conclusion: The findings revealed that GSH-Rd and CAT are the primary antioxidant defense markers in donkey red blood cells. The observed alterations in these principal antioxidants suggest a 14 days optimum keeping time of donkey blood for blood banking purposes. Keywords: blood, citrate phosphate dextrose adenine 1, donkey, malondialdehyde, superoxide dismutase.
Keywords: blood, citrate phosphate dextrose adenine 1, donkey, malondialdehyde, superoxide dismutase.
How to cite this article: Nnamdi OH, Ijeoma UR, Gilbert NL, Toochukwu EH, Ositadinma US (2020) In vitro assessment of time-dependent changes in red cell cytoplasmic antioxidants of donkey blood preserved in citrate phosphate dextrose adenine 1 anticoagulant, Veterinary World, 13(4): 726-730.
Received: 20-12-2019 Accepted: 09-03-2020 Published online: 19-04-2020
Corresponding author: Okereke Henry Nnamdi E-mail: nnamdi.okereke@unn.edu.ng
DOI: 10.14202/vetworld.2020.726-730
Copyright: Nnamdi, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.