Vet World Vol.13 April-2020 Article-20
Research Article
Veterinary World, 13(4): 751-757
https://doi.org/10.14202/vetworld.2020.751-757
Response of hemocytes profile in the black tiger shrimp (Penaeus monodon) against Vibrio harveyi induced by Xylocarpus granatum leaves extract
2. Laboratory of Experimental Pond, Faculty of Fisheries and Marine Sciences, Universitas Mulawarman. Jl. Gunung Tabur, Kampus Gunung Kelua, Samarinda 75124, East Kalimantan, Indonesia.
3. Department of Animal Science, Faculty of Agriculture, Universitas Mulawarman. Jl. Paser Balengkong, Kampus Gunung Kelua, Samarinda 75124, Indonesia.
Background and Aim: The present study investigated hemocytes profile of black tiger shrimp (Penaeus monodon) induced with Xylocarpus granatum leaves extract to protect against Vibrio harveyi infection.
Materials and Methods: X. granatum leaves were chopped into small size, air-dried, and extracted with one of the following solvents: Ethanol, distilled water, and seawater, whereas each solvent was given in three different concentrations (750 ppm, 1.000 ppm, and 1.250 ppm, respectively). Extracts were induced to 60 post-larvae shrimp in each treatment (three replicates, 20 shrimp for each) by immersing method and subsequently challenged with V. harveyi.
Results: This study demonstrated different effectiveness among solvents used to extract X. granatum leaves, in which distilled water showed the most effective solvent as can be seen from the lowest percentage on anorexia, lethargic, and weakened reflex of shrimp compared with another solvent, positive and negative controls. Pathological symptoms for shrimp induced by X. granatum leaves extract were minimum with the highest survival rate compared with those of positive and negative control. Total hemocyte cells and its cell constituents such as semi-granular, granular, and hyaline cells on treatment group at 1.250 ppm were higher than controls.
Conclusion: Leaves extract of X. granatum extract effectively inhibited V. harveyi infection, increased survival rate, and hemocytes cell of the experimental shrimp. Distilled water extract of X. granatum at 1.250 ppm demonstrated the highest protective effect toward V. harveyi infection on P. monodon. Keywords: hemocytes profile, mangrove plant extract, Penaeus monodon, Vibrio harveyi, Xylocarpus granatum.
Keywords: hemocytes profile, mangrove plant extract, Penaeus monodon, Vibrio harveyi, Xylocarpus granatum.
How to cite this article: Saptiani G, Sidik AS, Ardhani F, Hardi EH (2020) Response of hemocytes profile in the black tiger shrimp (Penaeus monodon) against Vibrio harveyi induced by Xylocarpus granatum leaves extract, Veterinary World, 13(4): 751-757.
Received: 20-11-2019 Accepted: 05-03-2020 Published online: 22-04-2020
Corresponding author: Gina Saptiani E-mail: gina_saptiani@yahoo.com
DOI: 10.14202/vetworld.2020.751-757
Copyright: Saptiani, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.