Research Article | 22 Apr 2020

Clinical results of an inactivated anti-brucella vaccine in combination with immunomodulators

Fayssal Bouchemla1, Valery Alexandrovich Agoltsov1, Stepan Yuryevich Veselovsky1, Sergey Vasilyevich Larionov1, Olga Mikhaylovna Popova2, and Dmitry Valentinovich Krivenko1Show more
VETERINARY WORLD | pg no. 758-763 | Vol. 13, Issue 4 | DOI: 10.14202/vetworld.2020.758-763
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Abstract

          
Background and Aim: The aim of the study was to obtain a vaccine against animal brucellosis having high immunogenic properties by carrying an evaluation of the effectiveness of split-conjugated animal brucellosis vaccine combined with fosprenil and polypeptide C as a molecular immunomodulatory adjuvant according to the results of serological studies of the blood of animals: Agglutination reaction, complement fixation, and rose Bengal sample.
Materials and Methods: Eighteen calves of Holstein Friesians breed, aged 5 months, with a living weight of 100-150 kg, were divided into three groups of six animals each. All animals were healthy and they received a prophylactic vaccination against brucellosis. The dry split-conjugated vaccine against brucellosis in animals was dissolved in saline and for this purpose, 10 ml of saline was poured into the vaccine vial. Then the content was mixed, and afterward 1 ml was used per animal. Fosprenil was used at the rate of 1 kg of animal weight: 100 kg (calf weight) was multiplied by 0.05 (dose/1 kg of animal weight); 5 ml of fosprenil was obtained, which was collected into disposable syringes and intramuscularly sterilely injected into the croup area.
Results: After conducting serological studies, it was noted that split-conjugated vaccine against animal brucellosis using fosprenil forms antibodies in large titers and they persist for a longer time in the body of animals compared to the other tested vaccine: The first combination with the immunomodulatory polypeptide C and the vaccine only on the physiological solution.
Conclusion: The developed complex of split-conjugated vaccine against brucellosis in animals enhances the humoral immune response of the organism against brucellosis and improves the protection of animals against the disease when it is used with the immunomodulatory fosprenil. In the future, we want to expand the use of the resulting complex in the fight against brucellosis on a larger population and to study the change in cellular immunity after the introduction of the resulting complex on an animal organism. Keywords: brucellosis, fosprenil, polypeptide C, split-conjugated vaccine against animal brucellosis.
          
Keywords: brucellosis, fosprenil, polypeptide C, split-conjugated vaccine against animal brucellosis.