Butylated hydroxytoluene protects bull sperm surface protein-P25b in different extenders following cryopreservation

Vet World Vol.13 April-2020 Article-6

Research Article

Veterinary World, 13(4): 649-654

https://doi.org/10.14202/vetworld.2020.649-654

A. M. Khumran¹, N. Yimer², Y. Rosnina², H. Wahid², M. O. Ariff³, H. Homayoun⁴, K. Asmatullah², and T. K. Bello⁵

1. Department of Theriogenology and Production, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria.
2. Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
3. Department of Veterinary Pre-clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
4. Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
5. Biotechnology Research Programme, National Animal Production Research Institute, Zaria, Nigeria.

Background and Aim: The aim of this study was to investigate the effects of different concentration of butylated hydroxytoluene (BHT) on sperm membrane surface protein "P25b" from cryopreserved bull semen in either lecithin based Bioxcell® (BX) or two egg-yolk based extenders, tris-egg yolk (TEY), and citrate-egg yolk (CEY).

Materials and Methods: Forty-five semen samples, 15 each were extended with either BX, TEY, or CEY extender which contained different concentrations (0.0 - control, 0.5, 1.0, 1.5, 2.0, and 3.0 mM/mL) of BHT. The extended semen samples were frozen at a concentration of 20×106/mL in 0.25 mL straws and stored in liquid nitrogen for 2 weeks. The frozen samples were thereafter thawed, proteins extracted and analyzed for quantities of protein P25b through direct sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel densitometry. Peptides were confirmed by Western blotting (WB).

Results: Results showed that supplementation of BHT improved (p<0.05) quantity of protein P25b at concentrations of 0.5 mM/mL for BX and at 1.0 mM/mL for TEY and CE when compared with the controls and other treatments.

Conclusion: BHT supplementation at 0.5 in BX and 1.0 mM/mL in TEY and CEY has protected bull sperm fertility marker protein P25b in frozen-thawed bull sperm. Keywords: bull sperm, butylated hydroxytoluene, extender, P25b, Western blotting.

Keywords: bull sperm, butylated hydroxytoluene, extender, P25b, Western blotting.

How to cite this article: Khumran AM, Yimer N, Rosnina Y, Wahid H, Ariff MO, Homayoun H, Asmatullah K, Bello TK (2020) Butylated hydroxytoluene protects bull sperm surface protein-P25b in different extenders following cryopreservation, Veterinary World, 13(4): 649-654.

Received: 06-08-2019 Accepted: 24-02-2020 Published online: 11-04-2020

Corresponding author: N. Yimer E-mail: nurhusien@upm.edu.my

DOI: 10.14202/vetworld.2020.649-654

Copyright: Khumran, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.