Tempe extract reduces cell damage in the liver and kidneys after intensive physical exercise in rats

Vet World Vol.13 August-2020 Article-3

Research Article

Veterinary World, 13(8): 1510-1516

https://doi.org/10.14202/vetworld.2020.1510-1516

I. Nyoman Suarsana¹, Iwan Harjono Utama¹, and I. Made Kardena²

1. Laboratory of Biochemistry, Faculty of Veterinary Medicine, Udayana University, Denpasar, Indonesia.
2. Laboratory of Pathology, Faculty of Veterinary Medicine, Udayana University, Denpasar, Bali, Indonesia.

Background and Aim: Cells of the liver and kidneys are perpetually exposed to free radicals from endogenous and exogenous sources. High-intensity physical exercise can induce oxidative stress. This study aimed to determine the effects of tempe extract on cell damage in the liver and kidneys of rats after intensive physical exercise.

Materials and Methods: This study used five experimental groups: T0 (non-exercised control rats), T1 (rats made to exercise by swimming), and T2-T4 (rats made to exercise by swimming treated with 25, 50, and 100 mg/kg body weight tempe extract). The biochemical parameters that were analyzed included blood glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), blood urea nitrogen (BUN), and creatinine levels. The morphology of liver and kidney tissues was histopathologically and immunohistochemically analyzed.

Results: Tempe extract treatment reduced cell damage in the liver and kidney tissues of rats, characterized by decreased expression of caspase-3. In addition, the ALT, AST, ALP, creatinine, and BUN levels of rats were significantly lower in tempe extract-treated rats than in rats after swimming exercise alone.

Conclusion: Tempe extract is capable of reducing cell damage and apoptosis in the liver and kidney cells of rats after intensive physical exercise and maintaining biochemical properties similar to the normal physiological state. Keywords: apoptosis, caspase-3, kidney, liver, physical exercise, tempe.

Keywords: apoptosis, caspase-3, kidney, liver, physical exercise, tempe.

How to cite this article: Suarsana IN, Utama IH, Kardena IM (2020) Tempe extract reduces cell damage in the liver and kidneys after intensive physical exercise in rats, Veterinary World, 13(8): 1510-1516.

Received: 23-02-2020 Accepted: 10-06-2020 Published online: 07-08-2020

Corresponding author: I. Nyoman Suarsana E-mail: suarsana65@unud.ac.id

DOI: 10.14202/vetworld.2020.1510-1516

Copyright: Suarsana, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.