Development of recombinant nucleocapsid protein-based indirect enzyme-linked immunosorbent assay for sero-survey of porcine reproductive and respiratory syndrome

Vet World Vol.13 December-2020 Article-2

Research Article

Veterinary World, 13(12): 2587-2595

https://doi.org/10.14202/vetworld.2020.2587-2595

S. Phani Kashyap^1,2, Jagadish Hiremath¹, S. Vinutha^1,2, Sharanagouda S. Patil¹, Kuralayanapalya P. Suresh¹, Parimal Roy¹, and Divakar Hemadri¹

1. ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, P.B. No. 6450, Yelahanka, Bengaluru, Karnataka, India.
2. Department of Microbiology and Biotechnology, Jain University, Bengaluru, Karnataka, India.

Background and Aim: Porcine reproductive and respiratory syndrome (PRRS) is a disease endemic in many countries and is of economic importance. India was free from PRRS until the first outbreak was reported from a North-East Indian state in 2013. Since then, disease outbreaks have been reported from North-East India and the pilot study conducted earlier showed that it is gradually spreading to the rest of India. Considering there are no locally developed population screening tests available for PRRS and imported diagnostic/screening tests are expensive, the present study was aimed at developing recombinant nucleocapsid (rN) protein-based indirect enzyme-linked immunosorbent assay (iELISA).

Materials and Methods: The rN protein of PRRS virus (PRRSV) was produced following standard cloning, expression, and purification procedures. Using this antigen, iELISA was optimized for the detection of serum antibodies to PRRSV. The sensitivity and specificity of the test were assessed by comparing it with a commercial PRRSV antibody detection kit.

Results: A total of 745 serum samples from ten different states of India were screened using the developed iELISA. The iELISA had a relative specificity of 76.18% and sensitivity of 82.61% compared to the commercial ELISA (Priocheck PRRSV ELISA kit, Thermo Fisher Scientific, USA).

Conclusion: The iELISA, which deployed rN protein from Indian PRRSV, was found to be suitable in the serological survey and may be a useful tool in future disease surveillance programs. Keywords: indirect enzyme-linked immunosorbent assay, nucleocapsid protein, porcine reproductive and respiratory syndrome, sensitivity, specificity.

Keywords: indirect enzyme-linked immunosorbent assay, nucleocapsid protein, porcine reproductive and respiratory syndrome, sensitivity, specificity.

How to cite this article: Phani Kashyap S, Hiremath J, Vinutha S, Patil SS, Suresh KP, Roy P, Hemadri D (2020) Development of recombinant nucleocapsid protein-based indirect enzyme-linked immunosorbent assay for sero-survey of porcine reproductive and respiratory syndrome, Veterinary World, 13(12): 2587-2595.

Received: 29-06-2020 Accepted: 21-10-2020 Published online: 05-12-2020

Corresponding author: Divakar Hemadri E-mail: divakar.hemadri@icar.gov.in

DOI: 10.14202/vetworld.2020.2587-2595

Copyright: Phani Kashyap, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.