Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells

Vet World Vol.13 December-2020 Article-24

Research Article

Veterinary World, 13(12): 2772-2779

https://doi.org/10.14202/vetworld.2020.2772-2779

Parishma Debbarma¹, Tanmay Mondal², Camelia Manna¹, Kuldeep Kumar², Joydip Mukherjee¹, Bikash Chandra Das³, Sadhan Bag², and Kinsuk Das¹

1. Department of Veterinary Physiology, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India.
2. Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
3. Eastern Regional Station, ICAR-Indian Veterinary Research Institute, Kolkata, West Bengal, India.

Background and Aim: Veterinary health care is an emergent area in animal sciences and innovative therapeutic approaches happen to be imperative in the present days. In view of the importance of cattle health and production, it is necessary to take up contemporary approach of stem cell therapy in this sector also. This study aimed to standardize an explant culture method of bovine umbilical tissue offcut to isolate mesenchymal stem cells (MSCs) because considerable efforts are required for ensuring easy accessibility and availability of MSCs in bulk quantity, as well as in establishing and characterizing the cell lines.

Materials and Methods: The umbilical cord (UC) tissue matrix offcut was collected after calving. A simplified in vitro cell isolation technique was followed to collect the emerged out cells from the explants of UC. Further, we expanded these isolated cells in vitro, observed its growth kinetics, and characterized to confirm as per the criterion of bovine MSCs.

Results: A considerable exponential growth rate of the UC-derived cells was noticed. In addition to their confirmation as MSCs, the cells also exhibited plastic adherent property and maintained the spindle-shaped morphology throughout the in vitro culture. The cultured cells were found positive MSC-specific surface markers CD105, CD90, and CD73 and were negative for hematopoietic cell marker CD45. Cytochemical studies revealed the ability of the cells to differentiate into osteogenic, chondrogenic, and adipogenic lineages.

Conclusion: This simplified method of isolation and culture of bovine multipotent MSCs from the UC offcut collected after calving could be extrapolated for the greater availability of the cells for prospective therapeutic applications. Keywords: cattle, umbilical cord tissue, mesenchymal stem cells, isolation.

Keywords: cattle, umbilical cord tissue, mesenchymal stem cells, isolation.

How to cite this article: Debbarma P, Mondal T, Manna C, Kumar K, Mukherjee J, Das BC, Bag S, Das K (2020) Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells, Veterinary World, 13(12): 2772-2779.

Received: 22-06-2020 Accepted: 16-11-2020 Published online: 25-12-2020

Corresponding author: Kinsuk Das E-mail: kin.suk@hotmail.com

DOI: 10.14202/vetworld.2020.2772-2779

Copyright: Debbarma, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.