Vet World Vol.13 February-2020 Article-22
Research Article
Veterinary World, 13(2): 386-391
https://doi.org/10.14202/vetworld.2020.386-391
Molecular characterization of sheeppox virus from outbreaks in Karnataka, India
2. Department of Microbiology and Biotechnology, Jain University, Bengaluru, Karnataka, India.
Background and Aim: This study aimed to characterize sheeppox virus (SPPV) using the P32 gene of the Capripoxvirus (CaPVs).
Materials and Methods: Clinical samples of skin, scabs, and nasal swab from suspected outbreaks Horalagallu (n=13) and Gerahalli (n=11) at Ramanagara district in Karnataka were collected. All the samples were initially subjected to genus-specific diagnostic polymerase chain reaction (PCR). The pooled clinical samples from each outbreak were also subjected to virus isolation. The isolates were confirmed by CaPVs genotyping PCR targeting the full-length P32 gene, followed by sequencing and phylogenetic analysis.
Results: The clinical signs and lesions varied from mild to severe degree with no specificity between age and sex. Specific cytopathic changes in cell morphology were observed in infected Vero cells from both outbreaks, which were confirmed by PCR. The complete P32 gene from two outbreaks was successfully amplified with the expected amplicon size of 1006bp. The sequencing and phylogenetic analysis revealed that both the outbreaks were due to SPPV and shared high similarity with published SPPVs from Karnataka and other parts of India.
Conclusion: The current study showed that complete P32 gene-based genotypic PCR assay can be used for genetic characterization and molecular epidemiology of both sheeppox and goatpox diseases and also to differentiate the causative agents. The sequence analysis revealed 100% similarity among the two outbreak isolates suggesting the same strain of the virus and common source of infection for the outbreaks. Keywords: goatpox, P32, polymerase chain reaction, phylogenetic analysis, sheeppox.
Keywords: goatpox, P32, polymerase chain reaction, phylogenetic analysis, sheeppox.
How to cite this article: Sumana K, Revanaiah Y, Apsana R, Roy P, Reddy GBM (2020) Molecular characterization of sheeppox virus from outbreaks in Karnataka, India, Veterinary World, 13(2): 386-391.
Received: 11-11-2019 Accepted: 16-01-2020 Published online: 28-02-2020
Corresponding author: G. B. Manjunatha Reddy E-mail: gbmpatho@gmail.com
DOI: 10.14202/vetworld.2020.386-391
Copyright: Sumana, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.