Molecular characteristic of Pasteurella multocida isolates from Sumba Island at East Nusa Tenggara Province, Indonesia

Vet World Vol.13 January-2020 Article-15

Research Article

Veterinary World, 13(1): 104-109

https://doi.org/10.14202/vetworld.2020.104-109

I. K. Narcana¹, I. W. Suardana², and I. N. K. Besung³

1. Master Student of Veterinary Medicine, Faculty of Veterinary Medicine, Udayana University, Jl. PB. Sudirman Denpasar-Bali, 80232, Indonesia.
2. Department of Preventive Veterinary Medicine, Laboratory of Veterinary Public Health, Faculty of Veterinary Medicine, Udayana University, Jl. PB. Sudirman Denpasar-Bali, 80232, Indonesia.
3. Department of Pathobiology, Laboratory of Veterinary Microbiology, Faculty of Veterinary Medicine, Udayana University, Jl. PB. Sudirman Denpasar-Bali, 80232, Indonesia.

Background and Aim: This study aimed to determine the molecular characteristics of Pasteurella multocida isolates originated from Sumba Island, East Nusa Tenggara Province.

Materials and Methods: The isolates of P. multocida stored in frozen storage were cultured in blood agar as a selective medium and identified conventionally. Molecular tests were initiated by DNA isolation and then followed by polymerase chain reaction tests with specific primers for the determination of P. multocida serotype A or B. Positive strain of serotype B was then confirmed molecularly using 16S rRNA gene primer and followed by the sequencing of nucleotides.

Results: The study showed that both P. multocida isolates from Sumba island, i.e. PM1 is isolated from East Sumba district, while PM2 isolated from West Sumba district have 99.6% homology. Both isolates also known have 99% similarities with P. multocida originated from India, Britain, and Japan, respectively. The isolates share the same clade in the phylogenetic tree.

Conclusion: The 16S rRNA sequencing revealed a high similarity of P. multocida serotype B:2 isolated from Sumba island with the Indian isolates although the sample size is very small. Therefore, further molecular studies like multilocus sequence typing, VNTR need to be performed using a larger number of samples to establish the genetic relatedness observed in this study. Keywords: genetic relatedness, molecular genetic, Pasteurella multocida, Septicemia Epizootica, Sumba island.

Keywords: genetic relatedness, molecular genetic, Pasteurella multocida, Septicemia Epizootica, Sumba island.

How to cite this article: Narcana IK, Suardana IW, Besung INK (2020) Molecular characteristic of Pasteurella multocida isolates from Sumba Island at East Nusa Tenggara Province, Indonesia, Veterinary World, 13(1): 104-109.

Received: 24-06-2019 Accepted: 25-11-2019 Published online: 13-01-2020

Corresponding author: I. W. Suardana E-mail: wayan_suardana@unud.ac.id

DOI: 10.14202/vetworld.2020.104-109

Copyright: Narcana, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.