Vet World Vol.13 January-2020 Article-7
Research Article
Veterinary World, 13(1): 47-53
https://doi.org/10.14202/vetworld.2020.47-53
Effect of Nauclea subdita (Korth.) Steud. leaf extract on hematological and histopathological changes in liver and kidney of striped catfish infected by Aeromonas hydrophila
2. Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Lambung Mangkurat University, Banjarbaru, South Kalimantan 70714, Indonesia.
3. Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Brawijaya University, Malang, East Java 65145, Indonesia.
Background and Aim: The present study was conducted to investigate the efficacy of different doses of Bangkal (Nauclea subdita) leaf extract on hematological and histological changes in kidney and liver of catfish (Pangasius hypophthalmus) infected by Aeromonas hydrophila.
Materials and Methods: Catfish were experimentally infected with A. hydrophila at a dose of 108 cells/mL through intraperitoneal injection, and the hematological and histological changes in the kidney and liver of catfish against the pathogen were observed.
Results: Not all concentrations of N. subdita caused a toxic effect in striped catfish. The clinical symptoms of catfish after infection with A. hydrophila and treatment with N. subdita leaf extract included morphological and behavioral changes. N. subdita leaf extract reduced mortality caused by A. hydrophila. Treatment with N. subdita leaf extract was effective in reducing the inflammation by decreasing the activity of neutrophils, monocytes, and lymphocytes. The Hb and Ht levels of catfish significantly decreased after exposure to 108 cells/mL of A. hydrophila and increased significantly after N. subdita treatment. Necrosis percentages in the kidney and liver also decrease after N. subdita treatment.
Conclusion: The results indicate that N. subdita leaf extract stimulates the immunity and increases the resistance of catfish to A. hydrophila. N. subdita leaf extract may be used as a potential source for future drug development and food applications. Keywords: Aeromonas hydrophila, hematology, histopathology, Nauclea subdita.
Keywords: Aeromonas hydrophila, hematology, histopathology, Nauclea subdita.
How to cite this article: Aisiah S, Prajitno A, Maftuch M, Yuniarti A (2020) Effect of Nauclea subdita (Korth.) Steud. leaf extract on hematological and histopathological changes in liver and kidney of striped catfish infected by Aeromonas hydrophila, Veterinary World, 13(1): 47-53.
Received: 14-08-2019 Accepted: 29-11-2019 Published online: 09-01-2020
Corresponding author: Siti Aisiah E-mail: sitiaisiah@ulm.ac.id
DOI: 10.14202/vetworld.2020.47-53
Copyright: Aisiah, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.