Sequencing and phylogenetic analysis of infectious bronchitis virus variant strain from an outbreak in egg-layer flocks in Baghdad, Iraq

Vet World Vol.13 July-2020 Article-15

Research Article

Veterinary World, 13(7): 1358-1362

https://doi.org/10.14202/vetworld.2020.1358-1362

Abdullah O. Alhatami¹, Furkan Alaraji², Husam Muhsen Abdulwahab², and Yahia Ismail Khudhair³

1. Department of Microbiology, Faculty of Veterinary Medicine, University of Kufa, Iraq.
2. Department of Pathology and Poultry Diseases, Faculty of Veterinary Medicine, University of Kufa, Iraq.
3. Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.

Background and Aim: Infectious bronchitis (IB) has an influential economic impact on the poultry industry, causing huge losses each year due to the condemnation of infected chickens. Despite the use of many kinds of vaccines in Iraq, it is common to find IB problems in vaccinated chickens. Information about the strains that affect Iraqi chickens is very limited. Therefore, we aimed to detect the currently circulating strains of IB virus that cause frequent outbreaks in egg layers despite the use of vaccination against the virus.

Materials and Methods: Isolate detection, sequencing, and phylogenetic analysis were performed using a rapid IB virus antigen kit (32 tracheal swabs), flinders technology associates (FTA) card (32 tracheal swabs), and partial gene sequencing (16 positive FTA samples).

Results: The isolated strain was different from other strains, especially the strain isolated in the North of Iraq (Sulemania Strain) and shares 98% homology with an Israeli strain (Israel variant 2, IS 1494).

Conclusion: Although more studies are needed to detect IB virus strains circulating in Iraq, this work lays the foundation for making a good strategy to control the disease and selecting vaccines that should be used in farms. Keywords: infectious bronchitis, phylogenetic tree, poultry, real-time polymerase chain reaction, spike 1 gene.

Keywords: infectious bronchitis, phylogenetic tree, poultry, real-time polymerase chain reaction, spike 1 gene.

How to cite this article: Alhatami AO, Alaraji F, Abdulwahab HM, Khudhair YI (2020) Sequencing and phylogenetic analysis of infectious bronchitis virus variant strain from an outbreak in egg-layer flocks in Baghdad, Iraq, Veterinary World, 13(7): 1358-1362.

Received: 05-02-2020 Accepted: 21-05-2020 Published online: 16-07-2020

Corresponding author: Furkan Alaraji E-mail: furkans.alaraji@uokufa.edu.iq

DOI: 10.14202/vetworld.2020.1358-1362

Copyright: Alhatami, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.