Vet World Vol.13 July-2020 Article-17
Research Article
Veterinary World, 13(7): 1372-1375
https://doi.org/10.14202/vetworld.2020.1372-1375
Isolation of peripheral blood mononuclear cells and the expression of toll-like receptors in Betong chickens
2. Department of Companion Animal Clinical Science, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
3. Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80161, Thailand.
Background and Aim: Toll-like receptors (TLRs) comprise microbial sensing receptors present on cell surfaces that are capable of detecting pathogens. The present study aims to examine the expression of TLRs within the peripheral blood mononuclear cell (PBMC) of the Betong chickens.
Materials and Methods: Blood samples were harvested from 12 Betong (KU line) chickens. Hematological values were calculated. PBMC was isolated from the blood utilizing a Histopaque solution and stored in a RPMI1640 culture medium. Cell viability was investigated using a Trypan Blue dye exclusion test. DNA was extracted from PBMC and the expression of the DNA's TLRs was examined using a polymerase chain reaction.
Results: Hematological values were determined from the blood samples collected in this study obtained from healthy Betong chickens. PBMC that was isolated from the Betong chickens possessed cell viability higher than 95% (95.37±1.06). From the examination of TLRs gene expression, results revealed instances of TLR1.1, TLR1.2, TLR2.1, TLR2.2, TLR3, TLR4, TLR5, TLR 7, TLR15, and TLR21 that were present in the PBMC of Betong chickens.
Conclusion: PBMC isolated from the blood of healthy Betong chickens possessed excellent cell quality. All chicken TLRs were discovered within the PBMC of Betong chickens. Hence, PBMC stands out as one of the premier sources for in vitro studies of chicken immune response. Keywords: Betong chicken, peripheral blood mononuclear cell, toll-like receptor.
Keywords: Betong chicken, peripheral blood mononuclear cell, toll-like receptor.
How to cite this article: Suklek A, Kayan A, Rattanasrisomporn J, Boonkaewwan C (2020) Isolation of peripheral blood mononuclear cells and the expression of toll-like receptors in Betong chickens, Veterinary World, 13(7): 1372-1375.
Received: 24-02-2020 Accepted: 19-05-2020 Published online: 18-07-2020
Corresponding author: Chaiwat Boonkaewwan E-mail: chaiwat.bo@wu.ac.th
DOI: 10.14202/vetworld.2020.1372-1375
Copyright: Suklek, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.