Antibacterial and cytotoxic activity assessment of Channa striatus (Haruan) extract

Vet World Vol.13 March-2020 Article-17

Research Article

Veterinary World, 13(3): 508-514

https://doi.org/10.14202/vetworld.2020.508-514

Nur Zulaikha Mat Zawawi¹, Rumaizi Shaari¹, Muhammad Luqman Nordin¹, Ruhil Hayati Hamdan², Tan Li Peng³, and C. W. Salma C. W. Zalati³

1. Department of Clinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Locked Bag 36, Pengkalan Chepa, 16100 Kota Bharu, Kelantan, Malaysia.
2. Department of Preclinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Locked Bag 36, Pengkalan Chepa, 16100 Kota Bharu, Kelantan, Malaysia.
3. Department of Paraclinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Locked Bag 36, Pengkalan Chepa, 16100 Kota Bharu, Kelantan, Malaysia.

Background and Aim: Channa striatus extract, a freshwater snakehead fish known as Haruan, is popular in Southeast Asia for consumption and as a traditional therapeutic remedy for wound healing. C. striatus is also used in osteoarthritic for its anti-inflammatory. The aim of this study was to determine the presence of antibacterial properties of C. striatus extract against oral bacteria and to investigate the cytotoxic activity against Vero cells.

Materials and Methods: The authors prepared C. striatus extract in chloroform-methanol solvents. Next, the authors took subgingival microbiological samples from 16 cats that had periodontal disease. The authors determined the antibacterial properties of C. striatus extract against the isolated bacteria using the disk diffusion method and a broth microdilution-based resazurin microtiter assay. Finally, the authors used the Vero cell line to evaluate the cytotoxic activity, and they assessed the cell availability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.

Results: The results showed weak antibacterial activity of C. striatus extract against Pseudomonas spp. and Escherichia coli. In addition, the authors found that minimum inhibition concentration values ranged between 400 and 500 mg/mL, and minimum bactericidal concentration values ranged between 650 and 550 mg/mL. However, the cytotoxic results were promising, showing that C. striatus extract increased the cell viability and growth when it was at a higher concentration. The extract also promotes growth and cell proliferation.

Conclusion: These findings suggest that C. striatus extract promoted cell proliferation in vitro and could be a plausible therapeutic wound healing alternative for periodontal disease in cats. Keywords: antibacterial properties, Channa striatus, feline periodontitis, periodopathogen.

Keywords: antibacterial properties, Channa striatus, feline periodontitis, periodopathogen.

How to cite this article: Zawawi NZM, Shaari R, Nordin ML, Hamdan RH, Peng TL, CWZalati CWS (2020) Antibacterial and cytotoxic activity assessment of Channa striatus (Haruan) extract, Veterinary World, 13(3): 508-514.

Received: 15-09-2019 Accepted: 28-01-2020 Published online: 19-03-2020

Corresponding author: Rumaizi Shaari E-mail: rumaizi@umk.edu.my

DOI: 10.14202/vetworld.2020.508-514

Copyright: Zawawi NZM, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.