Vet World   Vol.13   May-2020  Article-24

DNA of Brugia malayi detected in several mosquito species collected from Balangan District, South Borneo Province, Indonesia

Background and Aim: Lymphatic filariasis (LF) is a lesser-known parasitic disease, which contributes to significant decreases in overall health. This study investigated the presence of Brugia malayi in mosquitoes collected in the South Borneo Province, Indonesia.

Materials and Methods: Mosquitoes were collected through bare leg collection methods after sunset in several areas of the Hukai and Gulinggang villages in the Balangan District. The collected mosquitoes were identified based on morphological features and dissected to find microfilaria and then pooled through species for polymerase chain reaction (PCR) microfilaria detection.

Results: A total of 837 female mosquitoes consisting of at least 14 species were selected; they were dissected, and no microfilariae were found. Mosquitoes were divided into 69 pools for PCR analysis. PCR revealed that 8.7% (6/69) of the pools were positive for B. malayi, including Mansonia annulifera (4 pools), Aedes albopictus (1 pool), and Culex tritaeniorhynchus (1 pool).

Conclusion: These results suggested that mosquito dissection was not an optimum method for finding microfilaria. M. annulifera, C. tritaeniorhynchus, and A. albopictus mosquitoes might play an important role in the transmission of LF in the Balangan District. Information from this study could be used for the prevention of transmission or vector control programs in Indonesia. Keywords: Brugia malayi, lymphatic filariasis, polymerase chain reaction.

Keywords: Brugia malayi, lymphatic filariasis, polymerase chain reaction.

How to cite this article: Supriyono S, Tan S (2020) DNA of Brugia malayi detected in several mosquito species collected from Balangan District, South Borneo Province, Indonesia, Veterinary World, 13(5): 996-1000.

Received: 17-01-2020  Accepted: 21-04-2020     Published online: 30-05-2020

Corresponding author: Suriyani Tan   E-mail: suriyanitan@gmail.com

DOI: 10.14202/vetworld.2020.996-1000

Copyright: Supriyono and Tan, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.