Vet World Vol.13 May-2020 Article-27
Research Article
Veterinary World, 13(5): 1010-1016
https://doi.org/10.14202/vetworld.2020.1010-1016
Effect of pre-supplementation with Pleurotus sajor-caju crude extracts on body weight and consequence responses of leukocytes and immune organs in fancy carp following inoculation with Aeromonas veronii
2. Department of Microbiology, Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand.
Background and Aim: The present study aimed at highlighting the effects of oyster mushroom (Pleurotus sajor-caju), as a dietary supplement on growth performance, differential leukocytes population, and histological changes of melanomacrophage centers (MMCs) in spleen and kidney of fancy carp on bacterial infection.
Materials and Methods: A total of 60 fancy carp were allocated into four groups according to feed formulations including; (1) basal diet with 2% crude extract of P. sajor-caju, (2) basal diet with 2% β-glucan, whereas Group 3, and Group 4 were positive and negative control, which were fed only basal diet. Diets were provided for 30 days, thereafter, fish of Group 1 to Group 3 were intraperitoneally injected with Aeromonas veronii (1.8×109 CFU), whereas Group 4 was injected with normal saline. At day 7 post-bacterial inoculation, all fish were weighed, whole blood was collected for differential white blood cell count, and two visceral organs, posterior kidney and spleen, were collected from euthanized fish to observe histological changes, particularly MMCs.
Results: No significant differences in body weight were found (p>0.05) at 1st week of the experiment; however, fish body weight was significantly increased from week 2 to week 4 of the experiment. Increased monocyte number was found in carp fish fed with the P. sajor-caju or β-glucan supplemented diets compared to the control groups (p<0.05). The proliferation of monocyte in fish was consistent with increased number and size of MMCs in hemotopoietic organs, posterior kidney and spleen, especially in fancy carp fed with of P. sajor-caju crude extract and commercially available β-glucan before bacterial inoculation in fish.
Conclusion: These findings indicate that crude polysaccharide from P. sajor-caju can be potentially used as a feed additive that might promote innate immune function in fish. Keywords: Aeromonas veronii, body weight, fancy carp (Cyprinus carpio), immunostimulant, Pleurotus sajor-caju.
Keywords: Aeromonas veronii, body weight, fancy carp (Cyprinus carpio), immunostimulant, Pleurotus sajor-caju.
How to cite this article: Rattanachan S, Bunnajirakul S, Punyadarsaniya D (2020) Effect of pre-supplementation with Pleurotus sajor-caju crude extract on body weight and consequence response of leukocytes and immune organs in fancy carp following inoculation with Aeromonas veronii, Veterinary World, 13(5): 1010-1016.
Received: 29-01-2020 Accepted: 16-04-2020 Published online: 31-05-2020
Corresponding author: Sitthichon Rattanachan E-mail: rsitthic@mut.ac.th
DOI: 10.14202/vetworld.2020.1010-1016
Copyright: Rattanachan, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.