Black soybean tempeh and purple sweet potato improve sperm quality in streptozotocin-induced diabetic rats

Vet World Vol.13 November-2020 Article-33

Research Article

Veterinary World, 13(11): 2534-2540

https://doi.org/10.14202/vetworld.2020.2534-2540

Abdul Gofur¹, Agung Witjoro¹, Siti Nur Arifah¹, Mochammad Fitri Atho'illah², Yuslinda Annisa¹, and Sri Rahayu Lestari¹

1. Department of Biology, Faculty of Mathematics and Science, Universitas Negeri Malang, Jalan Semarang no. 5, 65145, Malang, East Java, Indonesia.
2. Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, East Java, Indonesia.

Background and Aim: Hyperglycemia increases advanced glycation end-product (AGE) production, and the activity of receptor for AGE (RAGE) in testis, which leads to testicular histopathological damage and infertility. This research investigated the effect of black soybean tempeh (BST), purple sweet potato (PSP), and its combination on AGE and RAGE expression and spermatozoa quality in streptozotocin (STZ)-induced diabetic rats.

Materials and Methods: The rats were given high-fat diets for 5 weeks, then were injected intraperitoneally with multiple low doses of STZ (30 mg/kg body weight). Diabetes mellitus (DM) rats were divided into seven groups: DM, DM+glibenclamide, DM+BST, DM+PSP, and DM+combination of BST and PSP in ratio 1:3, 2:2, and 3:1 as C1, C2, and C3, respectively. The rats were treated for 30 days. Testicular AGE and RAGE expression and spermatozoa quality were measured.

Results: The combination of BST and PSP significantly decreased AGE and RAGE expression in testicular organs and improved spermatozoa quality compared to the normal group.

Conclusion: The combination of BST and PSP can be used as future alternatives to improve spermatozoa quality in DM patients. Keywords: antioxidant, black soybean tempeh, purple sweet potato, spermatozoa.

Keywords: antioxidant, black soybean tempeh, purple sweet potato, spermatozoa.

How to cite this article: Gofur A, Witjoro A, Arifah SN, Atho'illah MF, Annisa Y, Lestari SR (2020) Black soybean tempeh and purple sweet potato improve sperm quality in streptozotocin-induced diabetic rats, Veterinary World, 13(11): 2534-2540.

Received: 19-06-2020 Accepted: 19-10-2020 Published online: 27-11-2020

Corresponding author: Sri Rahayu Lestari E-mail: srirahayulestari@um.ac.id

DOI: 10.14202/vetworld.2020.2534-2540

Copyright: Gofur, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.