Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line

Vet World Vol.14 April-2021 Article-22

Research Article

Veterinary World, 14(4): 964-971

https://doi.org/10.14202/vetworld.2021.964-971

Dolly Patricia Pardo-Mora¹, Oscar Julián Murillo², Mauricio Rey-Buitrago^1,2, Mónica Losada-Barragán¹, Jaime Fabian Cruz Uribe¹, Karina Basso Santiago³, Bruno José Conti³, Eliza de Oliveira Cardoso³, Fernanda Lopes Conte³, Rafael María Gutiérrez¹, Orlando Torres García¹, and José Maurício Sforcin³

1. Department of Animal Health, Facultad de Medicina Veterinaria, Universidad Antonio Nariño, Bogotá, Colombia.
2. Department of Morphology, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia.
3. Department of Chemical and Biological Sciences, São Paulo State University (UNESP), Institute of Biosciences, Campus Botucatu, Brazil.

Background and Aim: Osteosarcoma (OSA) is the most common bone tumor in canines and humans. This study aimed to assess the cytotoxic and apoptotic effects of Colombian propolis samples on a canine OSA cell line (OSCA-8) by evaluating the expression of BCL-2, BAX, CASPASE 9, CASPASE 8, and TNFR1 genes involved in the apoptosis pathway.

Materials and Methods: After treating the cells with five Colombian propolis samples (Usm, Met, Fus, Sil, and Caj), we evaluated cell viability and lactate dehydrogenase (LDH) release. Early and late apoptosis was determined by flow cytometry using annexin V/propidium iodide. Furthermore, the effects of three selected samples on gene expression were analyzed by real-time polymerase chain reaction.

Results: The Colombian propolis samples reduced OSCA-8 cell viability and increased LDH release. All samples induced apoptosis significantly and upregulated BCL-2 and CASPASE 8 expression. Usm and Sil increased BAX expression, Met and Sil induced CASPASE 9 expression, and Usm increased TNFR1.

Conclusion: Colombian propolis samples exhibited cytotoxic and apoptotic effects on canine OSA cells, and CASPASE 8 upregulation indicated apoptosis induction by the extrinsic pathway. Keywords: apoptosis, cytotoxicity, osteosarcoma, propolis.

Keywords: apoptosis, cytotoxicity, osteosarcoma, propolis.

How to cite this article: Pardo-Mora DP, Murillo OJ, Rey-Buitrago M, Losada-Barragán M, Uribe JFC, Santiago KB, Conti BJ, Cardoso EO, Conte FL, Gutiérrez RM, García OT, Sforcin JM (2021) Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line, Veterinary World, 14(4): 964-971.

Received: 08-10-2020 Accepted: 01-03-2021 Published online: 22-04-2021

Corresponding author: José Maurício Sforcin E-mail: jose.m.sforcin@unesp.br

DOI: 10.14202/vetworld.2021.964-971

Copyright: Pardo-Mora, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.