Vet World Vol.14 April-2021 Article-31
Research Article
Veterinary World, 14(4): 1028-1037
https://doi.org/10.14202/vetworld.2021.1028-1037
Comparative analysis of canine mesenchymal stem cells and bone marrow-derived mononuclear cells
2. Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoinooka, Imabari, Ehime, 794-8555, Japan.
3. Science and Humanities Master's Programme, Graduate School of Science and the Humanities, Kurashiki University of Science and The Arts, 2640 Nishinoura Tsurajima Kurashiki Okayama, 712-8505, Japan.
4. Department of Comparative Animal Science, College of Life Science, Kurashiki University of Science and The Arts, 2640 Nishinoura Tsurajima Kurashiki Okayama, 712-8505, Japan.
Background and Aim: Mesenchymal stem cells (MSCs), which have multi-lineage differentiation potentials, are a promising source for regenerative medicine. However, the focus of study of MSCs is shifting from the characterization of the differentiation potential to their secretion potential for cell transplantation. Tissue regeneration and the attenuation of immune responses are thought to be affected by the secretion of multiple growth factors and cytokines by MSCs. However, the secretion potential of MSCs profiling remains incompletely characterized. In this study, we focused on the secretion ability related and protein mRNA expression of dog adipose tissue-derived MSCs (AT-MSC), bone marrow (BM)-derived MSCs, and BM-derived mononuclear cells (BM-MNC).
Materials and Methods: Real-time polymerase chain reaction analyses revealed mRNA expression of nine growth factors and seven interleukins in these types of cells and three growth factors protein expression were determined using Enzyme-linked immunosorbent assay.
Results: For the BM-MNC growth factors, the mRNA expression of transforming growth factor-β (TGF-β) was the highest. For the BM-derived MSC (BM-MSC) and AT-MSC growth factors, the mRNA expression of vascular endothelial growth factor (VEGF) was highest. BM-MSCs and AT-MSCs showed similar expression profiles. In contrast, BM-MNCs showed unique expression profiles for hepatocyte growth factor and epidermal growth factor. The three types of cells showed a similar expression of TGF-β.
Conclusion: We conclude that expression of cytokine proteins and mRNAs suggests involvement in tissue repair and protection. Keywords: bone marrow-derived mononuclear cell, canine, growth factor, interleukin, mesenchymal stem cell, secretion.
Keywords: bone marrow-derived mononuclear cell, canine, growth factor, interleukin, mesenchymal stem cell, secretion.
How to cite this article: Maeta N, Tamura K, Ezuka F, Takemitsu H (2021) Comparative analysis of canine mesenchymal stem cells and bone marrow-derived mononuclear cells, Veterinary World, 14(4): 1028-1037.
Received: 27-10-2020 Accepted: 16-03-2021 Published online: 29-04-2021
Corresponding author: Hiroshi Takemitsu E-mail: takemitsu@kusa.ac.jp
DOI: 10.14202/vetworld.2021.1028-1037
Copyright: Maeta, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.