Comparison of enzyme-linked immunosorbent assay and Fassisi® bovine immunoglobulin G (IgG) immunoassay for quantification of bovine IgG in neonatal calf serum

Vet World Vol.14 December-2021 Article-20

Research Article

Veterinary World, 14(12): 3211-3215

https://doi.org/10.14202/vetworld.2021.3211-3215

Marian Hampe¹, Stefanie Söllner-Donat², Klaus Failing³, and Axel Wehrend¹

1. Clinic for Obstetrics, Gynecology, and Andrology of Large and Small Animals with Veterinary Ambulance, Justus-Liebig-University, D 35392 Giessen, Germany.
2. Animal Health Service, Thuringian Animal Diseases Fund, Jena, Germany.
3. Unit for Biomathematics and Data Processing, Justus-Liebig University, D 35392, Giessen, Germany.

Background and Aim: Rapid tests are routinely used to estimate serum immunoglobulin G (IgG) concentrations in diagnosing a failure of passive transfer (FPT) in calves. The study aimed to compare the Fassisi® Bovine IgG (FB-IgG) immunoassay and an enzyme-linked immunosorbent assay for quantifying bovine IgG in neonatal calf serum.

Materials and Methods: A total of 277 calves of 1-10 days of age were used in this study. Blood samples were obtained, and serum was extracted by centrifuging the samples at 2740× g for 5 min at 20°C. The serum was analyzed using the FB-IgG according to the manufacturer's specifications. Serum IgG concentrations were also determined by enzyme-linked immunosorbent assay (ELISA-IgG). FPT was defined as a serum IgG concentration <10 mg/mL.

Results: The mean ELISA-IgG serum concentration was 8.40 mg/mL (SD=7.02, range=0.10-47.50 mg/mL). FPT prevalence based on the ELISA measurements was 66.8%. The prevalence of partial and full FPT based on the FB-IgG was 54.5%. The ELISA-IgG and FB-IgG results were subjected to correlation and regression analysis. Overall sensitivity and specificity of the FB-IgG were 61.1% and 58.7%, respectively. A statistically significant dependence on age was identified in the results.

Conclusion: Our findings suggest that the FB-IgG rapid method is less accurate and provides no other advantages over established methods. Keywords: antibody concentration, calves, colostrum, passive transfer, rapid method.

Keywords: antibody concentration, calves, colostrum, passive transfer, rapid method.

How to cite this article: Hampe M, Söllner-Donat S, Failing K, Wehrend A (2021) Comparison of enzyme-linked immunosorbent assay and Fassisi

Received: 03-06-2021 Accepted: 08-11-2021 Published online: 30-12-2021

Corresponding author: Marian Hampe E-mail: marian.hampe@web.de

DOI: 10.14202/vetworld.2021.3211-3215

Copyright: Hampe, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.