Vet World Vol.14 February-2021 Article-17
Research Article
Veterinary World, 14(2): 452-456
https://doi.org/10.14202/vetworld.2021.452-456
Effect of adding growth factors during in vitro maturation on the developmental potentials of ewe oocytes selected by brilliant cresyl blue staining
2. Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Cairo University, Egypt.
Background and Aim: Several factors had been concerned with the developmental competence of the sheep oocyte. This study aims to investigate the effect of adding growth factors (insulin-like growth factor 1 [IGF-1] and epidermal growth factor [EGF]) in the maturation medium of ewe oocytes selected based on brilliant cresyl blue (BCB) screening on in vitro maturation (IVM), fertilization, and pre-implantation embryo development.
Materials and Methods: Cumulus-oocyte complexes (COCs) were obtained from the ovaries of slaughtered ewes by either aspiration or slicing techniques. COCs were in vitro matured in a medium containing IGF-1 and EGF (control group). For BCB screening, oocytes were stained and divided into BCB+ oocytes that matured in the same maturation conditions without adding growth factors (Group 2) or in the presence of growth factors (Group 3), and BCB– oocytes that matured in medium without growth factors (Group 4) or with growth factors (Group 5).
Results: The supplementation of the maturation medium with growth factors during IVM of (BCB+) oocytes resulted in a significant increase in nuclear maturation rate (90.9%), fertilization rate (75.6%), and embryo developmental rates (60.0%, 46.7%, and 33.3% for cleavage, morula, and blastocyst, respectively).
Conclusion: Culturing BCB+ oocytes in a maturation medium containing both EGF and IGF-1 showed a significant improvement in nuclear maturation, fertilization, and pre-implantation embryo development in vitro. Keywords: brilliant cresyl blue, embryo development, growth factors, In vitro fertilization, sheep.
Keywords: brilliant cresyl blue, embryo development, growth factors, In vitro fertilization, sheep.
How to cite this article: Fathi M, Elkarmoty AF (2021) Effect of adding growth factors during in vitro maturation on the developmental potentials of ewe oocytes selected by brilliant cresyl blue staining, Veterinary World, 14(2): 452-456.
Received: 12-09-2020 Accepted: 04-01-2021 Published online: 22-02-2021
Corresponding author: Amr F. Elkarmoty E-mail: amr.elkarmoty@yahoo.com
DOI: 10.14202/vetworld.2021.452-456
Copyright: Fathi and Elkarmoty, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.