Vet World Vol.14 January-2021 Article-16
Research Article
Veterinary World, 14(1): 120-128
https://doi.org/10.14202/vetworld.2021.120-128
Novel in vitro and in vivo anti-Helicobacter pylori effects of pomegranate peel ethanol extract
2. Department of Pharmacy, Faculty of Health Sciences, American University of Madaba, 11821 Madaba, Jordan.
3. Department of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of Jordan, Amman, Jordan.
4. Department of Pharmacology and Biomedical Sciences, Faculty of Pharmacy, University of Petra, 961343 Amman, Jordan.
Background and Aim: Interest in plants with antimicrobial properties has been revived due to emerging problems associated with using antibiotics to eradicate Helicobacter pylori. Accordingly, this study aims to assess the antibacterial effects of Punica granatum and the possible synergistic effect of its extract along with metronidazole against H. pylori.
Materials and Methods: Pomegranate peel ethanol extracts (PPEE) was tested against a control strain of H. pylori (NCTC 11916) in vitro and in vivo in female Wistar rats. Moreover, the synergistic effect of PPEE in combination with metronidazole was tested in vitro.
Results: The PPEE exhibited a remarkable activity against H. pylori with a minimum inhibitory concentration (MIC) of 0.156 mg/mL. Furthermore, the extract exhibited a pronounced urease inhibitory activity (IC50 ∼6 mg/mL) against the tested strain. A synergistic effect between PPEE and metronidazole was also observed (fractional inhibitory concentrations <0.5). Oral treatment of rats with PPEE for 8 days produced a significant reduction in H. pylori gastritis and a significant decrease in both lymphocytic and positive chronicity.
Conclusion: Pomegranate extract is probably safe and represents a potential alternative and complementary therapy for reducing H. pylori associated with gastric ulcers. Keywords: Helicobacter pylori, in vitro, in vivo, pomegranate peel ethanol extracts, urease inhibition.
Keywords: Helicobacter pylori, in vitro, in vivo, pomegranate peel ethanol extracts, urease inhibition.
How to cite this article: Mayyas A, Abu-Sini M, Amr R, Akasheh RT, Zalloum W, Khdair A, Hamad I, Aburjai T, Darwish RM, Abu-Qatouseh L (2021) Novel in vitro and in vivo anti-Helicobacter pylori effects of pomegranate peel ethanol extract, Veterinary World, 14(1): 120-128.
Received: 31-08-2020 Accepted: 01-12-2020 Published online: 16-01-2021
Corresponding author: Luay Abu-Qatouseh E-mail: labuqatouseh@uop.edu.jo
DOI: 10.14202/vetworld.2021.120-128
Copyright: Mayyas, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.