Vet World   Vol.14   July-2021  Article-23

Bacterial phagocytosis and reactive oxygen species production by camel neutrophils and monocytes are influenced by the type of anticoagulation agent

Background and Aim: Anticoagulants with different modes of action are used in the collection of camel blood samples. In the innate immune response, camel neutrophils and monocytes can play several roles during infection and inflammation. For anticoagulants ethylenediaminetetraacetic acid (EDTA) and heparin, research has described their effects on different parameters of the immune system. However, to date, no research has examined the effects of anticoagulants on the functional activity of camel phagocytes. Therefore, this study analyzed the influence of K3EDTA and lithium heparin on the antimicrobial activity of camel neutrophils and monocytes.

Materials and Methods: Camel leukocytes were separated from blood collected in EDTA or heparin tubes, and their phagocytosis and reactive oxygen species (ROS) production activity were analyzed by flow cytometry after stimulation with Staphylococcus aureus or Escherichia coli bacteria.

Results: In comparison to the cells collected from the EDTA blood, the camel neutrophils and monocytes separated from the heparin blood showed higher phagocytosis activity of S. aureus and E. coli. In addition, the neutrophils and monocytes produced significantly more ROS when the blood was collected in the heparin tubes.

Conclusion: The antimicrobial functions of camel neutrophils and monocytes are significantly affected by the type of anticoagulation agent. Therefore, using heparin rather than EDTA as an anticoagulant is recommended when performing the functional analysis of phagocytosis and ROS production of camel phagocytes. Keywords: bacteria, dromedary camel, monocyte, neutrophil, phagocytosis, reactive oxygen species.

Keywords: bacteria, dromedary camel, monocyte, neutrophil, phagocytosis, reactive oxygen species.

How to cite this article: Al Humam NA (2021) Bacterial phagocytosis and reactive oxygen species production by camel neutrophils and monocytes are influenced by the type of anticoagulation agent, Veterinary World, 14(7): 1888-1893.

Received: 28-03-2021  Accepted: 10-06-2021     Published online: 24-07-2021

Corresponding author: Naser Abdallah Al Humam   E-mail: nalhumam@kfu.edu.sa

DOI: 10.14202/vetworld.2021.1888-1893

Copyright: Al Humam, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.