Molecular identification of fluoroquinolone resistance in Salmonella spp. isolated from broiler farms and human samples obtained from two regions in Colombia

Vet World Vol.14 July-2021 Article-6

Research Article

Veterinary World, 14(7): 1767-1773

https://doi.org/10.14202/vetworld.2021.1767-1773

María Paula Herrera-Sánchez¹, Rafael Enrique Castro-Vargas^1,2, Luz Clemencia Fandiño-de-Rubio², Roy Rodríguez-Hernández², and Iang Schroniltgen Rondón-Barragán^1,2

1. Research Group in Immunobiology and Pathogenesis, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Santa Helena Highs, Ibagué 730006299, Tolima, Colombia.
2. Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Santa Helena Highs, Ibagué 730006299, Tolima, Colombia.

Background and Aim: Salmonella is one of the most common foodborne pathogens, the emergence of antibiotic-resistant strains of which is increasing. The aim of this study was to phenotypically and genotypically characterize the fluoroquinolone resistance of Salmonella isolates from broiler and humans in two regions of Colombia.

Materials and Methods: Salmonella strains (n=49) were evaluated. The phenotype of antibiotic resistance was assessed by an automated method and agar diffusion method, as well as the presence of the quinolone resistance genes qnrA, qnrB, qnrC, qnrD, qnrS, and aac(6')-Ib as determined by polymerase chain reaction.

Results: Strains were resistant to ciprofloxacin (75%), levofloxacin (57.1%), and enrofloxacin (38.8%). Molecular identification showed that 24 out of 49 strains possessed the qnrB gene (48.9%), while only one isolate from the Santander region possessed the aac(6')-Ib gene. Regarding Class 1 integron, it was present in 11 out of the 49 strains (22.44%).

Conclusion: This study reports the presence of the gene qnrB as well the presence of Class 1 integrons in broiler Salmonella isolates, which may contribute to the resistance to fluoroquinolones. Keywords: antibiotic resistance, broiler, resistance genes, Salmonella.

Keywords: antibiotic resistance, broiler, resistance genes, Salmonella.

How to cite this article: Herrera- Sánchez MP, Castro-Vargas RE, Fandiño-de-Rubio LC, Rodríguez-Hernández R, Rondón-Barragán IS (2021) Molecular identification of fluoroquinolone resistance in Salmonella spp. isolated from broiler farms and human samples obtained from two regions in Colombia, Veterinary World, 14(7): 1767-1773.

Received: 29-01-2021 Accepted: 24-05-2021 Published online: 07-07-2021

Corresponding author: Iang Schroniltgen Rondón-Barragán E-mail: isrondon@ut.edu.co

DOI: 10.14202/vetworld.2021.1767-1773

Copyright: Herrera- Sánchez, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.