Vet World Vol.14 June-2021 Article-11
Research Article
Veterinary World, 14(6): 1480-1486
https://doi.org/10.14202/vetworld.2021.1480-1486
Effect of Majapahit (Crescentia cujete L.) fruit powder on the immune profile of Litopenaeus vannamei after infection with Vibrio spp.
2. Department of Biology, Faculty of Mathematics and Natural Sciences, University of PGRI Ronggolawe, Tuban 62381, East Java, Indonesia.
3. Department of Biology Education, Faculty of Teacher Training and Education, University of PGRI Ronggolawe, Tuban 62381, East Java, Indonesia.
Background and Aim: The use of bioactive compounds is a promising tool to improve shrimp health regarding vibriosis. This study aimed to determine the effect of the dietary Majapahit (Crescentia cujete L.) fruit powder on the cellular immune response of vannamei shrimp (Litopenaeus vannamei) infected with Vibrio harveyi, Vibrio alginolyticus, and Vibrio parahaemolyticus.
Materials and Methods: Twelve vannamei shrimp (aged 1 month) were randomly divided into four groups (n=3). Vannamei shrimp received experimental feed with different concentrations of Majapahit fruit powder for 20 days: Group A, 0%; Group B, 3.04%; Group C, 6.08%, and Group D, 9.12%. Subsequently, vannamei shrimp was infected with V. harveyi, V. alginolyticus, and V. parahaemolyticus. The total hemocytes, total differential hemocytes (hyaline, semi-granular, and granular cells), and phagocytic activity were assessed. Data were analyzed through analysis of variance (p<0.05) using SPSS ver. 24 for Windows.
Results: Majapahit fruit powder at a dose of 3.04% increased the number of hyaline cells of L. vannamei after infection with Vibrio spp. Supplementation of the feed formula with Majapahit fruit powder at a dose of 3.04% increased the number of semi-granular and granular cells compared with the control. Furthermore, Majapahit fruit powder at doses of 3.04% and 6.08% increased the hemocytes compared with a dose of 9.12%. The phagocytic activity of L. vannamei after infection with Vibrio spp. tends to increase after supplementation with 3.04% Majapahit fruit powder.
Conclusion: This study showed that the addition of C. cujete L. powder to the feed formula increased the cellular immune response. The most effective dose ranged from 3.04% to 6.08%. Keywords: Crescentia cujete L., hemocytes, immune response, phagocytosis.
Keywords: Crescentia cujete L., hemocytes, immune response, phagocytosis.
How to cite this article: Rahmaningsih S, Andriani R, Pujiastutik H (2021) Effect of Majapahit (Crescentia cujete L.) fruit powder on the immune profile of Litopenaeus vannamei after infection with Vibrio spp., Veterinary World, 14(6): 1480-1486.
Received: 25-11-2020 Accepted: 19-04-2021 Published online: 10-06-2021
Corresponding author: Sri Rahmaningsih E-mail: rahmaningsihsri@unirow.ac.id
DOI: 10.14202/vetworld.2021.1480-1486
Copyright: Rahmaningsih, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.