Vet World   Vol.14   June-2021  Article-2

Effects of Gossypium spp., Balanites aegyptiaca, and Sesamum indicum seeds oils on quality of chilled and frozen-thawed ram semen

Background and Aim: Essential oils found frequently in plants are well known for their activities against bacteria, viruses, and fungi, and antioxidant properties. This study aimed to analyze egg yolk replacement by seed oils of Gossypium spp. (cotton), Balanites aegyptiaca (desert date), and Sesamum indicum (sesame) in semen extender, on ram sperm quality chilled at 4°C and frozen-thawed.

Materials and Methods: Ejaculates were collected from adult rams and refrigerated at 4°C in a Tris-based extender containing 1.25%, 2.5%, 5%, and 10% of Gossypium spp., B. aegyptiaca, and S. indicum seed oils, to evaluate which were the two best extenders for comparison with BIOXcell, a commercial extender for deep freezing ram semen.

Results: The data showed that sperm movements analyzed by the CASA system were faster in extenders supplemented with 2.5-5% of cottonseed oil and 1.25-10% of sesame oil, whereas in the extender containing B. aegyptiaca oil, all seminal parameters studied had the worst values. During the sperm-freezing process, 5% of cottonseed oil and 5% sesame seed oil were selected from the first study, with sesame oil reaching the best sperm quality. Thus, sperm motility and velocity were 44.14±13.99%, 24.44±12.6%, and 25.92±11.50%; and 20.26±9.56%, 8.76±6.38%, and 9.42±5.40%, respectively, for sesame oil, cottonseed oil, and BIOXcell.

Conclusion: In summary, 2.5-10% of cottonseed oil and 1.25-10% of sesame seed oil can replace egg yolk in a Tris–egg yolk–based extender. Moreover, a Tris-based extender supplemented with 5% sesame seed oil could be an alternative for deep freezing ram semen, even though these results need to be confirmed with semen collected from rams with appropriate sexual rest. Keywords: Balanites aegyptiaca, Gossypium spp., ram, semen freezing, Sesamum indicum.

Keywords: Balanites aegyptiaca, Gossypium spp., ram, semen freezing, Sesamum indicum.

How to cite this article: Akourki A, Echegaray A, Perdomo O, Escartin NA, Guillén M (2021) Effects of Gossypium spp., Balanites aegyptiaca, and Sesamum indicum seeds oils on quality of chilled and frozen-thawed ram semen, Veterinary World, 14(6): 1412-1419.

Received: 09-02-2021  Accepted: 15-04-2021     Published online: 03-06-2021

Corresponding author: Adamou Akourki   E-mail: a.akourki@univ-maradi.org

DOI: 10.14202/vetworld.2021.1412-1419

Copyright: Akourki, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.