Ameliorative effect of Mangosteen (Garcinia mangostana L.) peel infusion on the histopathological structures of the liver and kidney of rats (Rattus norvegicus Berkenhout, 1769) after H2O2 induction

Vet World Vol.14 June-2021 Article-22

Research Article

Veterinary World, 14(6): 1579-1587

https://doi.org/10.14202/vetworld.2021.1579-1587

J. R. A. Rusman¹, S. A. Sundari¹, A. Nuriliani², and H. T. Saragih²

1. Alumni of Faculty of Biology, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
2. Laboratory of Animal Structure and Development, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.

Background and Aim: Hydrogen peroxide (H2O2) is a free radical, widely use as a food preservative, may cause adverse effects to the body. Mangosteen contains various antioxidants may scavenge free radical produced by H2O2. This study examined the effects of mangosteen peel infusion on the liver and kidney of rats after H2O2 induction.

Materials and Methods: Thirty-six female Wistar rats were divided into six groups: Negative control, positive control (1% H2O2), as well as mangosteen peel infusion groups at a concentration of 0.25; 0.5; 1, and 2%. H2O2 induction was administered orally every day for 2 months followed by mangosteen peel infusion treatment (1 month) after H2O2 induction for 1 month. At the end of the experiment, the rats were sacrificed. The liver and kidney of each rat were collected for reactive oxygen species (ROS) and histopathological examinations. Furthermore, blood was collected for creatinine examination.

Results: H2O2 induction caused the highest ROS level elevation in the positive control group which was treated with H2O2 only. Meanwhile, 2% of mangosteen peel infusion returned the ROS levels to normal. It was also observed that mangosteen peel infusion restored creatinine levels to normal. Furthermore, 2% of mangosteen peel infusion improved the histopathological structure of the liver and kidneys after H2O2 induction.

Conclusion: Mangosteen peel infusion especially at a concentration of 2% has the potential to improve liver and kidney structure and functions after H2O2 induction. Keywords: antioxidant, female rat, H2O2, histological structure, mangosteen peel infusion.

Keywords: antioxidant, female rat, H2O2, histological structure, mangosteen peel infusion.

How to cite this article: Rusman JRA, Sundari SA, Nuriliani A, Saragih HT (2021) Ameliorative effect of Mangosteen (Garcinia mangostana L.) peel infusion on the histopathological structures of the liver and kidney of rats (Rattus norvegicus Berkenhout, 1769) after H

Received: 28-01-2021 Accepted: 06-05-2021 Published online: 18-06-2021

Corresponding author: H. T. Saragih E-mail: saragihendry@ugm.ac.id

DOI: 10.14202/vetworld.2021.1579-1587

Copyright: Rusman JRA, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.