Research Article | 13 May 2021

Identification and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia

Evi Nur Qolbaini1, Miftahudin Majid Khoeri2, Korrie Salsabila2, Wisiva Tofriska Paramaiswari2, Wisnu Tafroji2, I. Made Artika1,2, and Dodi Safari2Show more
VETERINARY WORLD | pg no. 1180-1184 | Vol. 14, Issue 5 | DOI: 10.14202/vetworld.2021.1180-1184
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Abstract

          
Background and Aim: Subclinical mastitis is an udder infection devoid of clinical symptoms, and Staphylococcus aureus is one of the bacteria causing this disease. This study aimed to identify and determine the prevalence and antibiotic susceptibility of methicillin-resistant S. aureus (MRSA)-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia.
Materials and Methods: S. aureus was isolated from subclinical mastitis milk specimens. All strains were confirmed by polymerase chain reaction-based detection of staphylococcal nuc, and MRSA was confirmed by the presence of mecA. Antibiotic susceptibility was determined using the disk diffusion method.
Results: From 86 milk samples, 49 isolates (57%) were confirmed as S. aureus. All S. aureus isolates were susceptible to tetracycline, gentamicin, chloramphenicol, erythromycin, and trimethoprim/sulfamethoxazole. Nine isolates were identified as MRSA (10.5%).
Conclusion: In this study, we reported MRSA-associated subclinical mastitis in Bogor, Indonesia. Keywords: Bogor, dairy cows, methicillin-resistant Staphylococcus aureus, subclinical mastitis.
          
Keywords: Bogor, dairy cows, methicillin-resistant Staphylococcus aureus, subclinical mastitis.