Vet World Vol.14 September-2021 Article-1
Research Article
Veterinary World, 14(9): 2296-2305
https://doi.org/10.14202/vetworld.2021.2296-2305
Molecular characterization and pathological identification of a novel strain of delta papillomavirus-4 (bovine papillomavirus-2) in Egypt
2. Genome Research Unit, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt.
3. Department of Pathology Research, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt.
4. Department of Biotechnology Research, Animal Health Research Institute, Agriculture Research Center (ARC), 12618 Dokki, Giza, Egypt.
5. General Organization of Veterinary Services, Dokki, Giza, Egypt.
Background and Aim: Bovine papillomaviruses (BPV) are a heterogeneous group of oncoviruses, distributed globally, which produce major economic losses. In the current study, we compared the results of different diagnostic approaches and compared the strains identified in this study with previously characterized strains at local and international levels.
Materials and Methods: Samples of skin warts were collected from five bovines with generalized papillomatosis from two Egyptian provinces, Menya and Ismailia, in 2020. Electron microscopy, molecular characterization, histopathological, and immunohistochemical examination were performed.
Results: BPV was detected using electron microscopy in the collected samples. Using molecular characterization, BPV-2 was successfully identified for 1st time in Egypt. The strain has 99.6% identity with the BPV-2 reference strains obtained from GenBank. These results were supported by histopathology and immunohistochemistry examination. Partial nucleotide sequences of the L1 gene were submitted to GenBank with accession numbers MW289843 and MW289844.
Conclusion: BPV-2 was reported for 1st time in the current study. The strain was identified grossly, microscopically, and pathologically and confirmed using molecular approaches. All results were consistent. The sequence analysis revealed that this strain has high sequence similarity to the reference Deltapapillomavirus-4, BPV-2 strains from Brazil and China. Keywords: bovine papillomaviruses-2, first detection, histopathology, immunohistochemistry, phylogeny, transmission electron microscopy.
Keywords: bovine papillomaviruses-2, first detection, histopathology, immunohistochemistry, phylogeny, transmission electron microscopy.
How to cite this article: Hassanien RT, Hamdy ME, Elnomrosy SM, Hussein HA, Afify AF, Darwish FM, Shehab G, Emran R, Abd-El-Moniem MII, Habashi AR, Fahmy HA, Ibraheem EM, Shahein MA, Attya M, Abdelhakim AMM, Hagag NM (2021) Molecular characterization and pathological identification of a novel strain of delta papillomavirus-4 (bovine papillomavirus-2) in Egypt, Veterinary World, 14(9): 2296-2305.
Received: 18-04-2021 Accepted: 26-07-2021 Published online: 03-09-2021
Corresponding author: Ahmed F. Afify E-mail: Fieldagy_ahmed@yahoo.com
DOI: 10.14202/vetworld.2021.2296-2305
Copyright: Hassanien, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.