Vet World Vol.15 August-2022 Article-23
Research Article
Veterinary World, 15(8): 2084-2088
https://doi.org/10.14202/vetworld.2022.2084-2088
Occurrence and biodiversity of Eimeria spp. (Apicomplexa: Eimeriidae) in Madura cattle reared on Kamal Subdistrict, Madura Island, Indonesia
2. Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
3. Indonesian Research Center for Veterinary Sciences, Indonesian Agency for Agricultural Research and Development, Ministry of Agriculture Republic Indonesia, Indonesia.
4. Department of Veterinary Medicine, School of Health and Life Sciences, Universitas Airlangga, Banyuwangi, Indonesia.
Background and Aim: In Indonesia, Madura cattle are native breeds that are expected to contribute to the improvement of regional meat self-sufficiency. Eimeria spp. are protozoans that are commonly found in ruminants. This study aimed to identify the occurrence and diversity of Eimeria spp. in Madura cattle.
Materials and Methods: In this study, fresh fecal samples were collected from 100 cattle in Kamal Subdistrict, Bangkalan District, Madura Island, Indonesia. Morphological detection was performed using a light microscope, and molecular identification was performed using a polymerase chain reaction. DNA amplification was conducted using various species-specific primers for Eimeria bovis, Eimeria zuernii, Eimeria auburnensis, Eimeria alabamensis, Eimeria ellipsoidalis, and Eimeria cylindrica.
Results: The results obtained 21% (21/100) of Eimeria spp. based on morphological detection. A total of 15 positive samples with 500–25,000/mL oocysts were selected for DNA extraction and amplification, resulting in 12 positive samples. Four Eimeria spp. were obtained based on molecular identification: E. bovis, E. zuernii, E. auburnensis, and E. cylindrica.
Conclusion: Four species of Eimeria namely E. bovis, E. zuernii, E. auburnensis, and E. cylindrica were identified from fecal sample of Madura cattle using PCR method in this study. Further comprehensive studies are required to investigate the pathogenicity of Eimeria spp. in Madura cattle. Therefore, improved and integrated management practices should be strengthened by local governments to prevent pathogenic diseases and increase national livestock productivity in Indonesia. Keywords: biodiversity, Eimeria species, infectious disease, Madura cattle, Madura Island.
Keywords: biodiversity, Eimeria species, infectious disease, Madura cattle, Madura Island.
How to cite this article: Hastutiek P, Lastuti NDR, Suwanti LT, Sunarso A, Kurniawati DA, and Yudhana A (2022) Occurrence and biodiversity of Eimeria spp. (Apicomplexa: Eimeriidae) in Madura cattle reared on Kamal Subdistrict, Madura Island, Indonesia, Veterinary World, 15(8): 2084–2088.
Received: 13-04-2022 Accepted: 01-08-2022 Published online: 27-08-2022
Corresponding author: Aditya Yudhana E-mail: adityayudhana@fkh.unair.ac.id
DOI: 10.14202/vetworld.2022.2084-2088
Copyright: Hastutiek, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.