Molecular characterization of genes responsible for biofilm formation in Staphylococcus aureus isolated from mastitic cows

Vet World Vol.15 January-2022 Article-26

Research Article

Veterinary World, 15(1): 205-212

https://doi.org/10.14202/vetworld.2022.205-212

Eman Shafeek Ibrahim¹, Amany Ahmed Arafa¹, Sohad Mohamed Dorgam¹, Rasha Hamdy Eid², Nagwa Sayed Atta¹, Wahid Hussein El-Dabae¹, and Eslam Gaber Sadek¹

1. Department of Microbiology and Immunology, National Research Centre, Dokki, Giza, Egypt.
2. Udder Health and Neonatal Disease, Animal Reproduction Research Institute, Giza, Egypt.

Background and Aim: Mastitis is considered a significant disease of lactating animals. There are new attitudes for recognizing genes responsible for causing this disease to overcome and change the manipulation of this problem. This study aimed to isolate and identify Staphylococcus aureus strains from mastitic bovine animals and detect some specific biofilm-forming genes (icaA, icaD, and biofilm-associated protein [bap] genes clfA, fnbA, agrI, agrII, agrIII, agrIV, and cna).

Materials and Methods: A total of 121 mastitic milk samples were analyzed using biochemical tests (catalase test, oxidative-fermentative test, and coagulase test) and Gram stain. Multiplex polymerase chain reaction was applied to characterize biofilm genes (icaA, icaD, bap, clfA, and fnbA) in addition to (agrI, agrII, agrIII, agrIV, and cna).

Results: Among the 121 milk samples, 35 staphylococci isolates were derived with an incidence of 28.92% (35/121); among them, 19 are coagulase positive. Ninety percent of the isolates had ica genes (icaA and icaD) while bap gene was not recognized in any isolate. In addition, the incidence of fnbA, can, and clfA was 89.5% each. The prevalence of agr specific groups (agrI, agrII, agrIII, and agrIV) was 78.9%, 52.6%, 10.5%, and 15.8%, respectively.

Conclusion: This study concluded that S. aureus has variant mechanisms of pathogenicity to form biofilm devoid of carrying a specific gene. Keywords: biofilm genes, mastitis, molecular identification, Staphylococcus aureus.

Keywords: biofilm genes, mastitis, molecular identification, Staphylococcus aureus.

How to cite this article: Ibrahim ES, Arafa AA, Dorgam SM, Eid RH, Atta NS, El-Dabae WH, Gaber ES (2022) Molecular characterization of genes responsible for biofilm formation in Staphylococcus aureus isolated from mastitic cows, Veterinary World, 15(1): 205-212.

Received: 06-09-2021 Accepted: 31-12-2021 Published online: 30-01-2022

Corresponding author: E-mail: dr_wahidhussein@yahoo.com

DOI: 10.14202/vetworld.2022.205-212

Copyright: Ibrahim, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.