Cystatin C assay validation using the immunoturbidimetric method to evaluate the renal function of healthy dogs and dogs with acute renal injury

Vet World Vol.15 June-2022 Article-25

Research Article

Veterinary World, 15(6): 1595-1600

https://doi.org/10.14202/vetworld.2022.1595-1600

Fabiola de Oliveira Paes-Leme¹, Eliana Matias de Souza¹, Mariah Gois Ceregatti¹, Marco Túlio Gomes Campos¹, Patricia Donado Vaz de Melo², and Adriane Pimenta da Costa-Val¹

1. Department of Clinics and Surgery, Veterinary School, Federal University of Minas Gerais, Belo Horizonte, Brazil.
2. Labtest Diagnostica SA, Lagoa Santa, Brazil.

Background and Aim: Acute kidney injury (AKI) is associated with a grave prognosis. A clinical assessment of kidney function can be performed based on the glomerular filtration rate (GFR). Cystatin C (CysC) can indicate the GFR or kidney function and its measurement is currently performed using immunological methods such as nephelometry, immunoturbidimetry, and enzyme-linked immunosorbent assays in human medicine. However, these techniques are not specific for use in veterinary medicine. This study aimed to validate an immunoturbidimetric assay for serum CysC (sCy) in dogs, determine the sCy reference intervals for healthy dogs, evaluate sCy stability in serum samples, and compare sCy with serum creatinine (sCr) in healthy dogs and dogs with AKI.

Materials and Methods: Forty-three dogs were divided into a control group (n = 19) and an AKI group (n = 24). An immunoturbidimetric method including commercially available human CysC calibrated with canine CysC was used to evaluate canine serum samples.

Results: An average recovery of 97% was observed for canine serum samples. The reference interval for CysC in healthy dogs was 0.57–1.29 mg/L. The sCy concentration in dogs with AKI was significantly higher (2.82 ± 1.46 mg/L) than in healthy dogs (0.93 ± 0.18 mg/L). Statistical analysis confirmed a strong correlation between sCy and sCr (r = 0.94; p < 0.05) in dogs with AKI.

Conclusion: The immunoturbidimetric method of evaluating sCy yielded satisfactory results and can be used for canine samples when a species-specific calibrator is used. Furthermore, sCy is a reliable marker of renal dysfunction in dogs. It is best to store samples for sCy evaluation at temperatures between 4°C and 8°C. Keywords: canine, immunoturbidimetry, kidney.

Keywords: canine, immunoturbidimetry, kidney.

How to cite this article: Paes-Leme FO, de Souza EM, Ceregatti MG, Campos MTG, Vaz de Melo PD, da Costa-Val AP (2022) Cystatin C assay validation using the immunoturbidimetric method to evaluate the renal function of healthy dogs and dogs with acute renal injury, Veterinary World, 15(6): 1595–1600.

Received: 05-11-2021 Accepted: 17-05-2022 Published online: 30-06-2022

Corresponding author: Fabiola de Oliveira Paes-Leme E-mail: fabiolapaesleme@vetufmg.edu.br

DOI: 10.14202/vetworld.2022.1595-1600

Copyright: Paes-Leme, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.