A review on the evolution of methods for intestinal in vitro organ culture and its application in veterinary science

Vet World Vol.16 February-2023 Article-14

Review Article

Veterinary World, 16(2): 347-356

https://doi.org/10.14202/vetworld.2023.347-356

Barbara Ribeiro de Souza Cortez^1,2 and Roberto Maurício Carvalho Guedes¹

1. Department of Veterinary Clinic and Surgery, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
2. Department of Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Canada.

Background and Aim: Different techniques have been reported in studies of intestinal in vitro organ culture (IVOC). A robust compilation of all available methods is lacking in the literature, making it difficult to choose a method that corresponds to the study's demands. In this review, readers can assess the most available methods, allowing them to evaluate which is more suitable for their purposes and requirements. A simplified view of culturing intestinal explants is presented, highlighting the approachability of IVOC. Relevant findings from diverse veterinarian studies, where explants played a major role, as well as the technique used in each, are described to illustrate its applications. Finally, the strengths and limitations of the innovative intestinal IVOC methods are discussed. This review provides a collection of methods for intestinal explant culture and their possible applications in veterinary research. In this way, it aims to broaden access to IVOC techniques and aid decision-making regarding the best suited for a study's purposes.

Keywords: enteropathogens, explants culture, intestinal pathogens, swine colon, ussing chamber.

How to cite this article: Cortez BRS and Guedes RMC (2023) A review on the evolution of methods for intestinal in vitro organ culture and its application in veterinary science, Veterinary World, 16(2): 347–356.

Received: 01-09-2022 Accepted: 06-01-2023 Published online: 21-02-2023

Corresponding author: Roberto Maurício Carvalho Guedes E-mail: guedes@vet.ufmg.br

DOI: 10.14202/vetworld.2023.347-356

Copyright: Cortez BRS and Guedes RMC, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.