Founding of the culture collection of antibiotic-resistant strains of zoonotic bacteria in the Russian Federation

Research (Published online: 19-07-2023)

11. Founding of the culture collection of antibiotic-resistant strains of zoonotic bacteria in the Russian Federation

Olga Ivanova, Dmitry Blumenkrants, Ekaterina Krylova, Irina Soltynskaya, Anastasia Goncharova, Evgeny Chaikin, Anna Akhmetzyanova, and Alexander Panin

Veterinary World, 16(7): 1451-1460

Olga Ivanova: Federal State Budgetary Institution, The Russian State Center for Animal Feed and Drug Standardization and Quality, Moscow, Russia.

Dmitry Blumenkrants: Federal State Budgetary Institution, The Russian State Center for Animal Feed and Drug Standardization and Quality, Moscow, Russia.

Ekaterina Krylova: Federal State Budgetary Institution, The Russian State Center for Animal Feed and Drug Standardization and Quality, Moscow, Russia.

Irina Soltynskaya: Federal State Budgetary Institution, The Russian State Center for Animal Feed and Drug Standardization and Quality, Moscow, Russia.

Anastasia Goncharova: Federal State Budgetary Institution, The Russian State Center for Animal Feed and Drug Standardization and Quality, Moscow, Russia.

Evgeny Chaikin: Federal State Budgetary Institution, The Russian State Center for Animal Feed and Drug Standardization and Quality, Moscow, Russia.

Anna Akhmetzyanova: Federal State Budgetary Institution, The Russian State Center for Animal Feed and Drug Standardization and Quality, Moscow, Russia.

Alexander Panin: Federal State Budgetary Institution, The Russian State Center for Animal Feed and Drug Standardization and Quality, Moscow, Russia.

doi: 10.14202/vetworld.2023.1451-1460

Article history: Received: 14-11-2022, Accepted: 29-05-2023, Published online: 19-07-2023

Corresponding author: Dmitry Blumenkrants

E-mail: d.blumenkrantz@vgnki.ru

Citation: Ivanova O, Blumenkrants D, Krylova E, Soltynskaya I, Goncharova A, Chaikin E, Akhmetzyanova A, and Panin A (2023) Founding of the culture collection of antibiotic-resistant strains of zoonotic bacteria in the Russian Federation, Veterinary World, 16(7): 1451-1460.

Abstract

Background and Aim: The main purpose of a national bioresource center is to standardize, centralize, preserve, and ensure accessibility of microbial bioresources that accumulate there because of state research programs. The establishment of national bioresource centers for antibiotic-resistant microorganisms allows to solve practical problems in the field of veterinary service, as well as to develop effective chemotherapeutic and disinfectant drugs to overcome the mechanisms of resistance. This study aimed to outline the process of forming a national culture collection of antibiotic-resistant strains of zoonotic bacteria in the Russian Federation using two microbial strains.

Materials and Methods: The object of research was isolates of Salmonella spp., Escherichia coli, Enterococcus spp., Campylobacter spp., Listeria monocytogenes, and Staphylococcus spp., all of which were obtained from biomaterials of farm animals, feed samples, bedding, water from livestock buildings, washouts from environmental objects, and food products. The resistance of bacterial isolates was determined using microbiological and molecular-genetic research methods.

Results: During monitoring studies, 1489 bacterial isolates were isolated. In total, 408 bacterial isolates were tested for sensitivity to antimicrobial agents, including E. coli (47.6%), Salmonella spp. (30.4%), Enterococcus spp. (11.3%), and Campylobacter spp. (10.8%). For genetic characterization, 95 isolates of Salmonella enterica, E. coli, Campylobacter spp., L. monocytogenes, Staphylococcus spp., Enterococcus spp. were chosen from the research collection, which was formed as part of the monitoring program for antibiotic resistance.

Conclusion: Deposited isolates that underwent whole-genome analysis can be used as positive control samples both in the development and use of methods or test systems for the detection of various resistance genes in zoonotic bacteria. In addition, such isolates can also be used for microbiological studies related to determining the sensitivity of microorganisms to antibacterial drugs, for phenotypic studies in the diagnosis of various bacterial infections in animals and birds, and retrospective analysis of strains from numerous collections.

Keywords: antibiotics, bioresource centers, genes, microorganisms, prevalence, resistance.