Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing Escherichia coli from chicken farms in Egypt

Vet World Vol.16 May-2023 Article-12

Research Article

Veterinary World, 16(5): 1001-1007

https://doi.org/10.14202/vetworld.2023.1001-1007

Gamal A. Salem¹, El-Sayed A. Abdelaziz¹, Mohammed A. Kamel¹, Nasreddin R. Rhouma², and Reem I. Ali¹

1. Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.
2. Department of Microbiology, Faculty of Science, Misurata University, Misurata 2478, Libya.

Background and Aim: Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli strains exhibit antibiotic resistance and are known to infect humans worldwide. This study assessed the phenotypic and genotypic prevalence of ESBL-resistant E. coli isolates recovered from the respiratory tracts of chickens in El-Sharkia Governorate, Egypt.

Materials and Methods: We obtained 250 lung samples (one lung/bird) from 50 chicken farms (5 chickens/farm) to isolate, identify, and serotype E. coli. Antimicrobial resistance susceptibility was determined using the disk diffusion method, while the ESBL phenotype was identified using double disk synergy. We detected the β-lactamase genes, blaTEM, and blaSHV, using a polymerase chain reaction.

Results: The results showed that 140/250 (56%) were infected with E. coli. All the serogroups of isolated E. coli exhibited high multi-antimicrobial resistance index values (>0.2), and 65.7% were confirmed to have ESBL. Among the isolates with the ESBL phenotypes, 55 (60%) and 32 (35%) contained the blaTEM and blaSHV genes, respectively.

Conclusion: The widespread distribution of multidrug-resistant and ESBL-producing E. coli among poultry farms is a significant human health hazard. These results will help the Egyptian authorities to implement a national one-health approach to combat the antimicrobial resistance problem. Keywords: antimicrobial resistance, β-lactamase, chicken farms, Escherichia coli.

Keywords: antimicrobial resistance, β-lactamase, chicken farms, Escherichia coli.

How to cite this article: Salem GA, Abdelaziz EA, Kamel MA, Rhouma NR, and Ali RI (2023) Prevalence of multidrug-resistant and extended-spectrum β–lactamase-producing

Received: 24-12-2022 Accepted: 03-04-2023 Published online: 13-05-2023

Corresponding author: E-mail: gamal_vet_85@yahoo.com

DOI: 10.14202/vetworld.2023.1001-1007

Copyright: Salem, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.