Artemisia vulgaris anthelmintic activities to ova and adult stages of Fasciola gigantica in vitro

Research (Published online: 30-05-2023)

29. Artemisia vulgaris anthelmintic activities to ova and adult stages of Fasciola gigantica in vitro

Andini Nurlaelasari, A’isyah Retno Wulandari, Tamara Muñoz Caro, Herjuno Ari Nugroho, Sukaryo Sukaryo, Muhammad Cahyadi, Wahyu Kurniawan, and Penny Humaidah Hamid

Veterinary World, 16(5): 1141-1153

Andini Nurlaelasari: Department of Animal Science, Faculty of Agriculture, Sebelas Maret University, Indonesia.

A’isyah Retno Wulandari: Department of Animal Science, Faculty of Agriculture, Sebelas Maret University, Indonesia.

Tamara Muñoz Caro: Escuela de Medicina Veterinaria, Facultad de Medicina Veterinaria y Recursos Naturales, Universidad Santo Tomás, Chile.

Herjuno Ari Nugroho: National Research and Innovation Agency, Indonesia.

Sukaryo Sukaryo: Department of Animal Science, Faculty of Agriculture, Sebelas Maret University, Indonesia.

Muhammad Cahyadi: Department of Animal Science, Faculty of Agriculture, Sebelas Maret University, Indonesia.

Wahyu Kurniawan: Agency of Livestock and Fishery Services, Boyolali District, Central Java, Indonesia.

Penny Humaidah Hamid: Department of Animal Science, Faculty of Agriculture, Sebelas Maret University, Indonesia

doi: 10.14202/vetworld.2023.1141-1153

Article history: Received: 12-01-2023, Accepted: 28-04-2023, Published online: 30-05-2023

Corresponding authors: Penny Humaidah Hamid

E-mail: pennyhumaidahhamid@staff.uns.ac.id

Citation: Nurlaelasari A, Wulandari AR, Caro TM, Nugroho HA, Sukaryo S, Cahyadi M, Kurniawan W, and Hamid PH (2023) Artemisia vulgaris anthelmintic activities to ova and adult stages of Fasciola gigantica in vitro, Veterinary World, 16(5): 1141-1153.

Abstract

Background and Aim: Fasciolosis due to Fasciola gigantica is endemic to tropical countries and Fasciola hepatica in temperate climates, highly detrimental to livestock and known as foodborne zoonotic diseases. The strategic control of the disease is mainly the use of chemical anthelmintic. This study aimed to evaluate the anthelmintic properties of Artemisia vulgaris extract on the ova and adult stages of F. gigantica.

Materials and Methods: Samples were collected from the Ampel Abbatoir, Boyolali District, Central Java, Indonesia. The ova from 20-gallbladders of cattle which were naturally infected with F. gigantica and 270 living F. gigantica worms were used in this study. The ovicidal assay was performed by incubating the ova with A. vulgaris in different concentrations, that is, 5%, 2.5%, and 1.25% for 5, 9, 11, 14, and 16 days. The efficacies were evaluated by quantification of ova degeneration during developmental stages in different time points and egg-hatch assay. The flukicidal effects were observed by mortality assay in 5, 10, 20, 40, 80, 160, 320, and 640 min incubations followed by scanning electron microscopy for surface morphology and histology of the fluke’s transversal sections.

Results: The concentration of 5% A. vulgaris showed the strongest ovicidal activities. The percentage of hatching ova on day 16 at concentrations of 5%, 2.5%, and 1.25% were 3.33%, 6.67%, and 16.67%. These ova hatch assay showed a significant reduction (p < 0.001) compared to untreated control. The flukicidal effect was significant (p < 0.001) at a concentration of 20%, with a mortality rate reaching 66.67% in the 40 min of incubation time. The surface properties of the adult worms, including the spine, tegument, acetabulum, intestine, and vitelline follicles, were disintegrated.

Conclusion: The results showed that A. vulgaris has the potential ovicidal and flukicidal properties to F. gigantica. The active compounds remained necessary to be elucidated further and its modes of action would be interesting to be predicted by molecular docking modeling.

Keywords: anthelmintic, Artemisia vulgaris, Fasciola gigantica, flukicidal, ovicidal.