Vet World Vol.17 April Article - 4
Research Article
Veterinary World, 17(4): 763-770
https://doi.org/10.14202/vetworld.2024.763-770
Genetic diversity and haplotype analysis of cattle hydatid cyst isolates using mitochondrial markers in Kazakhstan
1 Laboratory of Biodiversity and Genetic Resources, National Center for Biotechnology, Astana, Kazakhstan.
2 Laboratory of Parasitology, Department of Veterinary Medicine, S. Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan.
3 Department of Molecular Biology and Genetics, al-Farabi Kazakh National University, Almaty, Kazakhstan.
4 Laboratory of Parasitology and Mycology, Kazakh Scientific Research Veterinary Institute, Almaty, Kazakhstan.
5 Department of Microbiology and Virology, Astana Medical University, Astana, Kazakhstan.
6 Department of Scientific and Analytical Work, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan.
Background and Aim: In Kazakhstan, the study of Echinococcus infection among farm animals is crucial to monitor the invasion among livestock and map the data obtained. Unfortunately, there are only partial data on the study of Echinococcus among cattle's in Kazakhstan, which makes it difficult to conduct a comparative analysis of the epidemiological situation among livestock animals. The present study aimed to molecularly identify the species and haplotypes of the E. granulosus complex infecting cattle in Kazakhstan and investigate their genetic variation relative to mitochondrial (mt) targets.
Materials and Methods: Individual cyst isolates (n = 700) were collected from infected cattle lungs and livers after slaughter from the slaughterhouse. Total DNA was extracted from the germinal layers of the cyst from each isolate. This DNA sequenced partial mt genes of cytochrome c oxidase 1 (450 bp) and NADH dehydrogenase 1 (1200 bp).
Results: We determined that all the sequences were detected as E. granulosus s.s., of which 69 (94.5%) samples belonged to G1, and only 4 (5.4%) samples belonged to the G3 genotype. After bioinformatic analysis, 38 haplotypes were identified.
Conclusion: Our findings revealed that the G1 genotype of E. granulosus s.s. is the predominant cattle genotype in Kazakhstan. However, only one region showed the presence of two genotypes G1 and G3, in the sequence, which suggests that further research is needed to investigate the epidemiology of Echinococcus infection in cattle in Kazakhstan.
Keywords: cattle, cystic echinococcosis, Echinococcus granulosus, genotypes, haplotype, Kazakhstan.
How to cite this article: Uakhit R, Yalysheva S, Abdybekova A, Smagulova A, Lider L, Jazina K, Tautanova A, and Kiyan V (2024) Genetic diversity and haplotype analysis of cattle hydatid cyst isolates using mitochondrial markers in Kazakhstan, Veterinary World, 17(4):763-770.
Received: 31-10-2023 Accepted: 12-03-2024 Published online: 07-04-2024
Corresponding author: E-mail:
DOI: 10.14202/vetworld.2024.763-770
Copyright: Uakhit, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
