Vet World Vol.17 April Article - 9
Research Article
Veterinary World, 17(4): 804-810
https://doi.org/10.14202/vetworld.2024.804-810
Comparison of lipopolysaccharide-mediated peripheral blood mononuclear cell activation between Brahman and Brahman x Thai native crossbreed cattle
1 Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham 44000, Thailand.
2 Bioveterinary Research Unit, Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham 44000, Thailand.
3 Stress and Oxidative Stress in Animal Research Unit, Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham 44000, Thailand.
4 Department of Parasitology and Infectious Diseases, Gifu University Graduate School of Medicine, Gifu 5011194, Japan.
5 Department of Veterinary Technology, Faculty of Agriculture Technology, Kalasin University, Kalasin 46000, Thailand.
Background and Aim: Lipopolysaccharide (LPS) is a robust endotoxin known to activate the immune system in cattle. The objective of this study was to investigate the effect of LPS on the morphology, cell viability, malondialdehyde (MDA), nitric oxide (NO), and total antioxidant capacity (TAC) of peripheral blood mononuclear cells (PBMCs) in Brahman and Brahman x Thai native crossbreed cattle.
Materials and Methods: PBMCs were isolated from Brahman and Brahman x Thai native crossbreed cattle and treated with 0, 0.1, 1, and 10 μg/mL Escherichia coli LPS, respectively. Morphological changes in PBMCs were assessed at 24 and 48 h. In addition, we measured PBMC cell viability, MDA, NO, and TAC.
Results: LPS stimulation caused cell deformation and partial PBMC area enlargement, but there were no differences between Brahman and Brahman x Thai native crossbreed cattle. Stimulation at all levels did not affect the viability of PBMCs (p > 0.05). MDA and NO levels were significantly higher in Brahman cattle than in Brahman Thai native crossbred cattle (p < 0.05). TAC was significantly higher in Brahman x Thai native crossbred cattle than in Brahman cattle (p < 0.05).
Conclusion: Immune cells of crossbreed cattle have a higher activation response to LPS than those of purebred cattle, and native crossbreed beef cattle have a higher antioxidant capacity than purebred beef cattle. This result may explain why hybrid cattle of indigenous breeds are more resistant to disease than purebred cattle.
Keywords: Brahman cattle, lipopolysaccharide, oxidative stress, peripheral blood mononuclear cells, Thai native crossbreed cattle.
How to cite this article: Srinontong P, Aengwanich W, Somphon S, Khonwai S, Nitsinsakul T, Wu Z, Chalalai T, Saraphol B, and Srisanyong W (2024) Comparison of lipopolysaccharide-mediated peripheral blood mononuclear cell activation between Brahman and Brahman x Thai native crossbreed cattle, Veterinary World, 17(4): 804-810.
Received: 07-12-2023 Accepted: 21-03-2024 Published online: 10-04-2024
Corresponding author: E-mail:
DOI: 10.14202/vetworld.2024.804-810
Copyright: Srinontong, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
