Vet World   Vol.17   February-2024  Article-20

Biological activity comparison between ciprofloxacin loaded to silica nanoparticles and silver nanoparticles for the inhibition of Brucella melitensis

Background and Aim: Brucella melitensis is responsible for brucellosis, a highly contagious, life-threatening disease that has a high impact in low- and middle-income countries. This study aimed to compare silica nanoparticles (SiO-NPs) loaded with ciprofloxacin with silver nanoparticles (AgNPs) loaded with ciprofloxacin to evaluate the possible replacement of silver by silica to enhance biological activity and reduce cytotoxicity.

Materials and Methods: SiO-NPs and AgNPs loaded with ciprofloxacin were characterized using ultraviolet spectroscopy, scanning electron microscopy, and dynamic light scattering microscopy for size demonstration and loading efficiency. Both nanoparticles were treated with B. melitensis Rev 1 to evaluate their biological activity. Nanoparticle toxicity was also evaluated using cytotoxicity and hemolysis assays.

Results: SiO-NP was found to have a smaller size (80 nm) and higher loading efficiency with polydispersity index and zeta potential of 0.43 and 30.7 mV, respectively, compared to Ag-NP (180 nm and 0.62 and 28.3 mV, respectively). SiO-NP was potent with a minimum inhibitory concentration of 0.043 μg/mL compared to Ag-NP (0.049 μg/mL), with a lower cytotoxicity and hemolysis activity.

Conclusion: SiO-NP, as a drug delivery system for ciprofloxacin, has better antimicrobial activity against B. melitensis with lower cytotoxicity and hemolysis activity. These results can be attributed to the enhanced physical characterization and better loading efficiency when compared to Ag-NP. Keywords: antimicrobial activity, brucellosis, minimum inhibitory concentration, nanoparticles.

Keywords: antimicrobial activity, brucellosis, minimum inhibitory concentration, nanoparticles.

How to cite this article: Jaber SA, and Saadh MJ (2024) Biological activity comparison between ciprofloxacin loaded to silica nanoparticles and silver nanoparticles for the inhibition of Brucella melitensis, Veterinary World, 17(2): 407–412.

Received: 26-10-2023  Accepted: 23-01-2024     Published online: 20-02-2024

Corresponding author: Saif Aldeen Jaber   E-mail: sjaber@meu.edu.jo

DOI: 10.14202/vetworld.2024.407-412

Copyright: Jaber and and Saadh, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.