Effect of supersaturated dissolved oxygen on growth-, survival-, and immune-related gene expression of Pacific white shrimp (Litopenaeus vannamei)

Research (Published online: 08-01-2024)

6. Effect of supersaturated dissolved oxygen on growth-, survival-, and immune-related gene expression of Pacific white shrimp (Litopenaeus vannamei)

Songwut Patkaew, Sataporn Direkbusarakom, Ikuo Hirono, Suwit Wuthisuthimethavee, Sorawit Powtongsook, and Chettupon Pooljun

Veterinary World, 17(1): 50-58

Songwut Patkaew: Center of Excellence for Aquaculture Technology and Innovation, School of Agricultural Technology and Food Industry, Walailak University, Nakhon Si Thammarat, Thailand.

Sataporn Direkbusarakom: Center of Excellence for Aquaculture Technology and Innovation, School of Agricultural Technology and Food Industry, Walailak University, Nakhon Si Thammarat, Thailand.

Ikuo Hirono: Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan.

Suwit Wuthisuthimethavee: Center of Excellence for Aquaculture Technology and Innovation, School of Agricultural Technology and Food Industry, Walailak University, Nakhon Si Thammarat, Thailand.

Sorawit Powtongsook: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand; Department of Marine Science, Center of Excellence for Marine Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.

Chettupon Pooljun: Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat, Thailand; Research Center on One Health, Walailak University, Nakhon Si Thammarat, Thailand.

Background and Aim: Oxygen concentration is an essential water quality parameter for aquaculture systems. Recently, supersaturated dissolved oxygen (DO) has been widely used in aquaculture systems to prevent oxygen depletion; however, the long-term effects of supersaturated oxygen exposure on aquatic animals have not been studied. In this study, we examined the effects of supersaturated DO on the growth, survival, and gene expression of Pacific white shrimp (Litopenaeus vannamei).

Materials and Methods: Specific pathogen-free shrimp with a body weight of 8.22 ± 0.03 g were randomly assigned to two groups with four replicates at a density of 15 shrimps per tank. Shrimp were cultivated in recirculating tanks containing 50 L of 15 ppt seawater in each replicate. Oxygen was supplied at 5 mg/L to the control tanks using an air microbubble generator and at 15 mg/L to the treatment tanks using a pure oxygen microbubble generator. Shrimp were fed commercial feed pellets containing 39% protein at 4% of their body weight per day for 30 days. Average daily growth (ADG) and feed conversion ratio (FCR) were determined on days 15 and 30. Shrimp molting was measured every day. Individual hemolymph samples were obtained and analyzed for total hemocyte count, differential hemocyte count, and expression of growth- and immune-related genes at the end of the experiment.

Results: Long-term exposure to supersaturated DO significantly affected shrimp growth. After 30 days of supersaturated DO treatment, the final weight and ADG were 14.73 ± 0.16 g and 0.22 ± 0.04, respectively. Shrimp treated with normal aeration showed significantly lower weight (12.13 ± 0.13 g) and ADG (0.13 ± 0.00) compared with the control group. FCR was 1.55 ± 0.04 in the treatment group and 2.51 ± 0.09 in the control group. Notably, the shrimp molting count was 1.55- fold higher in the supersaturated DO treatment than in the supersaturated DO treatment. The expression of growth-related genes, such as alpha-amylase, cathepsin L, and chitotriosidase, was 1.40-, 1.48-, and 1.35-fold higher, respectively, after supersaturated DO treatment. Moreover, the treatment increased the expression of anti-lipopolysaccharide factor, crustin, penaeidin3, and heat shock protein 70 genes by 1.23-, 2.07-, 4.20-, and 679.04-fold, respectively, compared to the controls.

Conclusion: Supersaturated DO increased growth and ADG production and decreased FCR. Furthermore, enhanced immune-related gene expression by supersaturated DO may improve shrimp health and reduce disease risk during cultivation.

Keywords: gene expression, growth performance, molting, pacific white shrimp, supersaturated dissolved oxygen.