Vet World Vol.17 May-2024 Article - 6
Research Article
Veterinary World, 17(5): 981-987
https://doi.org/10.14202/vetworld.2024.981-987
Isolation and genetic characterization of waterfowl parvovirus in ducks in Northern Vietnam
2. Department of Veterinary Public Health, Faculty of Veterinary Medicine, Vietnam National University of Agriculture, 12400, Hanoi, Vietnam.
3. Department of Microbiolgy – Infectious disease, Faculty of Veterinary Medicine, Vietnam National University of Agriculture, 12400, Hanoi, Vietnam.
Background and Aim: Short beak and dwarfism syndrome (SBDS), a highly contagious disease, has been reported in duck farms in Vietnam since 2019. In this study, we evaluated the virulence and characterized the virus obtained from SBDS cases in North Vietnam.
Materials and Methods: Polymerase chain reaction was used to detect waterfowl parvovirus in ducks, and the virus from positive samples was inoculated into 10-day-old duck-embryonated eggs to reproduce the disease in young ducklings to determine the virulence and subjected to phylogenetic analysis of non-structural (NS) and VP1 gene sequences.
Results: Goose parvovirus (GPV) was isolated from ducks associated with SDBS in Vietnam. The virus Han-GPV2001 is highly virulent when inoculated into 10-day-old duck embryos and 3-day-old ducklings. The mortality rate of duck embryos was 94.35% within 6 days of virus inoculation. Inoculating 3-day-old ducks with the virus stock with 104.03 EID50 through intramuscular and neck intravenous administration resulted in 80% and 66.67% of clinical signs of SDBS, respectively, were shown. Phylogenetic analysis based on the partial NS and VP1 gene sequences revealed that the viral isolate obtained in this study belonged to novel GPV (NGPV) and was closely related to previous Vietnamese and Chinese strains.
Conclusion: A GPV strain, Han-GPV2001, has been successfully isolated and has virulence in duck-embryonated eggs as well as caused clinical signs of SBDS in ducks. Phylogenetic analyses of partial genes encoding NS and capsid proteins indicated that the obtained GPV isolate belongs to the NGPV group.
Keywords: Goose parvovirus, isolation, phylogenetic analysis, Vietnam, virulence.
How to cite this article: Huong NT, Hieu DV, Bich NT, Khanh TV, Ba NT, Xuan CTN, Hien QTM, Thai TH, and Huong CTT (2024) Isolation and genetic characterization of waterfowl parvovirus in ducks in Northern Vietnam, Veterinary World, 17(5): 981-987.
Received: 2024-01-23 Accepted: 2024-04-15 Published online: 2024-05-04
Corresponding author: E-mail:
DOI: 10.14202/vetworld.2024.981-987
Copyright: Huong, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
