Vet World Vol.18 March-2025 Article - 3
Research Article
Veterinary World, 18(3): 558-564
https://doi.org/10.14202/vetworld.2025.558-564
Gene expression profiles of angiogenesis markers and microRNA-128 from the secretome of umbilical cord mesenchymal stem cells from Macaca fascicularis
2. Center for Biomedical Research, National Research and Innovation Agency (BRIN), Cibinong Science Centre, Jalan Raya Bogor KM 46, Cibinong, West Java, Indonesia 16911.
3. Bio Farma Stem Cell Research and Development, Bandung, Indonesia 40161.
4. Department of Neurology, Faculty of Medicine, Dr. Hasan Sadikin Central General Hospital/Universitas Padjadjaran, Bandung, Indonesia 40161.
5. Department of Neurosurgery, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
6. Division of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, West Java, Indonesia.
Background and Aim: Angiogenesis and anti-apoptosis play crucial roles in ischemic stroke recovery. The mesenchymal stem cell (MSC) secretome, rich in bioactive molecules, presents a promising therapeutic avenue. However, optimizing the culture conditions to enhance the expression of angiogenic markers remains a challenge. This study examines the effect of hypoxic preconditioning on the expression of vascular endothelial growth factor (VEGF), monocyte chemoattractant protein-1 (MCP-1), matrix metalloproteinase-2 (MMP-2), and microRNA (miRNA-128) in the secretome of umbilical cord-derived MSCs (UC-MSCs) from Macaca fascicularis.
Materials and Methods: UC-MSCs were cultured under normoxic (21% O2) and hypoxic conditions (1%, 3%, and 5% O2) for 48 h. The secretome was isolated, and reverse transcription-quantitative polymerase chain reaction was used to quantify the expression of VEGF, MCP-1, MMP-2, and miRNA-128. Expression levels were normalized to housekeeping genes and analyzed using statistical methods to determine significant differences among groups.
Results: Hypoxic preconditioning significantly upregulated VEGF (1% O2), MCP-1 (5% O2), and miRNA-128 (5% O2) expression compared to normoxic conditions. Conversely, MMP-2 expression was highest in normoxic conditions and downregulated under hypoxia. In addition, miRNA-128 was found to be predominantly secreted into the extracellular space under hypoxic conditions rather than retained within cells.
Conclusion: Hypoxic preconditioning effectively modulates the expression of key angiogenesis and anti-apoptotic markers in UC-MSCs. The study highlights the importance of optimizing oxygen levels to enhance the therapeutic potential of MSCderived secretomes for ischemic stroke treatment. Future research should focus on in vivo validation and clinical translation of these findings.
Keywords: angiogenesis, hypoxic preconditioning, ischemic stroke, mesenchymal stem cells, microRNA-128, secretome.
How to cite this article: Utama HAN, Mariya SS, Rinendyaputri R, Dumingan A, Purwaningtyas YR, Intan PR, Renitia GI, Idrus HH, Ayuningtyas W, Noverina R, Huda F, Faried A, Sunarno A, and Malik A (2025) Gene expression profiles of angiogenesis markers and microRNA-128 from the secretome of umbilical cord mesenchymal stem cells from Macaca fascicularis, Veterinary World, 18(3): 558-564.
Received: 2024-11-22 Accepted: 2025-02-04 Published online: 2025-03-09
Corresponding author: E-mail:
DOI: 10.14202/vetworld.2025.558-564
Copyright: Utama, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
