Veterinary World

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May - 2010


Original Research

1.  Characteristics of Staphylococci isolated from mastitic goat milk in Iranian dairy herds - Azizollah Ebrahimi, Naser Shams, Somayeh Shahrokh and Pezhman Mirshokraei
Vet World. 2010; 3(5): 205-208

 

One hundred and fifteen cases of sub clinical mastitis were detected in a study on 510 goats from 5 herds in west central, Iran. From positive milk samples, 23 Staphylococcus spp. strains were isolated. Fourteen isolates were determined as Staphylococcus aureus (12.17%), and the other 9 (7.82%) as Staphylococcus epidermidis. Eleven and six isolates of S. aureus and S. epidermidis produced combined form of hemolysins a/ß/d, while ß/d hemolysins produced by 2 and 3 isolates respectively . Only one isolate of S. aureus produced single type d hemolysin. The sensitivity of all strains to 10 chemotherapeutics was tested through the disk diffusion method, 6 strains (26.08%) were determined as methicillin-resistant: out of them 4 isolates were S. aureus and 2 S.epidermidis. S. aureus and S. epidermidis isolates were 100% resistant to Cloxaciline and Kanamycin while the resistance to Penicillin was 100% in S. aureus and 33.33% in S. epidermidis isolates.
Keywords: Goat, Mastitis, Staphylococci, Hemolysin, Antimicrobial susceptibilities, Iran.


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Original Research

2.  Evaluation of pre and post artificial insemination effect of GnRH hormone on conception of repeat breeder Deoni cows - Mahesh S. Dodamani, Khaja Mohteshamuddin, S.D. Awati, M.K. Tandle and S.S. Honnappagol
Vet World. 2010; 3(5): 209-211


Twenty four Deoni repeat breeder cows were randomly allocated into 4 groups of six each. The animals of groups I, II and III were injected with 250 µg of buserelin acetate (Receptal®) on two occasions i.e. once on day of estrus and second dose on days 10 or 12 or14 respectively in I, II and III groups following breeding, while the animals of group IV served as control. Among the physical characters of estrual cervico-vaginal mucous, typical arborization pattern (80.95 % in pregnant vs. 55.56 % in non-pregnant cows) and marginally high spinnbarkeit readings (24.67+2.7cms in pregnant and 22.21+1.32 cms in non-pregnant cows) favored better fertility, although the differences between the groups were statistically insignificant. However, the pH of estrual cervico-vaginal mucous did not indicate any effect on fertility and it ranged between 8.00 to 9.00. The cows of treatment groups I, II and III registered a considerably higher conception rate of 83.33 percent each, while in control group cows had only 33.33 percent. To conclude GnRH therapy irrespective of days of administration resulted in an overall enhancement in conception rate of 83.33 as against 33.33 percent in control groups of cows.
Key Words: Repeat breeding, GnRH, Conception Rate, Cervical mucous and Deoni Cows.


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Original Research

3.  Comparision of polymerase chain reaction and agar gel immunodiffusion test in detection of Marek’s Disease virus - K. Jayalakshmi, G. Selvaraju, A. Manicavasaka Dinakaran, T.R. Gopalakrishna Murthy, M. Geetha and S. Saravanan
Vet World. 2010; 3(5): 212-214


A study was undertaken to identify Marek’s disease virus (MDV) antigen by PCR and AGID and to test the significance of PCR and AGID by McNemar’s test in detection of MDV antigen in outbreak in layer flocks. A total of twelve different MD outbreak flocks with varying flock size were selected in this study. Feather follicles were collected from 10 apparently healthy birds, 10 clinically affected birds and 10 dead birds separately in each outbreak. All the samples were subjected to PCR and AGID. In PCR, 42 (35.00%), 68 (56.67%) and 106 (88.33%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively and in AGID 28 (23.33%), 56 (46.67%) and 98 (81.67%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively. In testing the significance of PCR and AGID in detecting MDV, significant difference existed between the two tests in feather tips of apparently healthy birds (P < 0.05), whereas there was no significant difference between PCR and AGID in detection of MDV in feather tips of clinically affected and dead birds (P > 0.05). Hence, PCR can be used to screen MDV in apparently healthy birds and AGID can be used to screen MDV in clinically affected and dead birds keeping feasibility and economic consideration.
Keywords: Marek’s disease, Herpesvirus of turkey, Polymerase chain reaction, Agar gel immunodiffusion test


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Original Research

4.  Socio-economic status of livestock farmers of Narasapura village - A benchmark analysis - K. Sathyanarayan, V. Jagadeeswary, V. Chandrashekhar Murthy, S. Wilfred Ruban and G. Sudha
Vet World. 2010; 3(5): 215-218

 

The study was conducted following exploratory research design to ascertain the profile characteristics of livestock farmers. Findings indicated that majority of the farmers had low to medium profile. Hence efforts should be undertaken by the Government, Veterinary Universities and other extension agencies in providing information on livestock farming practices so that they could bring about change in their living and improve the socio-economic status of livestock farmers.
Keywords: Livestock Farmer, Socio-economic change, Analysis, Veterinary Extension.


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Original Research

5.  Incidence of canine hip dysplasia : A survey of 272 cases - Shiju Simon, M., R. Ganesh, S. Ayyappan, G. D. Rao, R. Suresh Kumar, M. Manonmani and B. C. Das
Vet World. 2010; 3(5): 219-220


A total of 272 cases of hip dysplasia were reviewed. A review of clinical cases presented with the clinical signs of hip dysplasia were referred to Radiology Unit of Madras Veterinary College, from May 2007-April 2009 was taken for this study.The incidence was highest in young animals of age group over three months to one year (52.94 percent). The breed-wise incidence was more common in Labrador Retriever (36.76 percent). Male dogs were found to be more affected (59.55 percent) than female dogs. Bilateral hip dysplasia was found to be more (88.60 percent) than unilateral. Among the unilateral hip dysplasia, left side was found to be more (54.83 percent) than right.
Keywords: Hip Dysplasia, Nutrition Imbalance, Canine, Radiology.


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Original Research

6.  Hormonal and biochemical profiles in follicular fluid of unovulated follicles in superovulated goats ovaries - S. B. Deshpande and M. M. Pathak
Vet World. 2010; 3(5): 221-223


The composition of follicular fluid (FF) varies with the cyclical hormonal changes and developmental stage of follicle. During superovulation programme, the large number of unovulated follicles is a major constraint and affects adversely the embryo recovery. An attempt was made to study the hormonal and biochemical profiles of unovulated follicles in goat superovulated with either Synthetic FSH (133 mg Folltropin) or 1000 IU PMSG (Folligon). The study revealed that Progesterone and testosterone concentrations were significantly (P<0.05) higher in FF of unovulated follicles of suparovulated goats as compared to control. The total and free cholesterol levels were significantly (P<0.05) lower in FF of treated animals then control one. The Alkaline Phosphosphatase activity was recorded lower in treated group while Acid Phosphosphatase activity was observed significantly (P<0.05) high in FF of treated animals compared to control. Iron and zinc concentration were also recorded significantly (P<0.05) higher in FF of superovulated animal compared to control. The total protein concentration was observed high in FF of treated animals than control but the differences were statistically non significant. The concentration of estradiol 17 ß, Lactate dehydrogenase and Copper did not show much variation between treatment and control group.
Key words: Goat, Superovulation, Unovulated Follicles, Follicular Fluid Composition


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Original Research

7.  Economic evaluation of milk cake with different packaging materials - S. N. Landge, D. N. Kulkarni and S. M. Gaikwad
Vet World. 2010; 3(5): 224-226


The ten samples of milk cake were prepared with various combinations and levels of ingredients such as sugar, deshi ghee and coagulant. Out of these samples only top five of them were selected for the cost of production, on the basis of ten point hedonic scale. And then out of these top five combinations, the sample required highest cost for production was selected to estimate the cost of production by using the packaging materials. for P1,P4 and P5 samples, Rs. 91.47 for P3 and P8, within the process combinations it is seen that with increased level of deshi ghee, the cost proportionately increased (i.e. in P3 and P8 samples). The non-dairy product such as sugar and alum also play a very important role to reduce the cost and increase the profit. Sugar contributes about 140 gm of weight in one kg. of product and cost just Rs.2.80 which helped to increase return from product because it is cheaper than the solids in the milk. The product sold in market at the rate of Rs. 110 per kg. which required net total cost for production about Rs.79.54 and Rs.82.04 without packaging material and with packaging material respectively.
Keywords:- Milk cake, cost of production, Polypropylene bag, Aluminium foil with LDPE laminates, Wax coated paper and Craft paper


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Original Research

8.  Genetic and non-genetic factors affecting body weight of buffaloes - Shashi Shankar and K.G. Mandal
Vet World. 2010; 3(5): 227-229

 

The present experiment was conducted on 60 randomly selected dairy units consisting of 116 Graded Murrah,70 Diara type and 121 Non-descript type buffalo cows utilizing the procedure of ‘’stratified random sampling with proportional allocation (Snedecor & Cochran,1967) in and around Patna.Genetic factors were the three different genetic groups of buffaloes viz. Graded murrah,Diara and Non-descript types prevalent in Bihar.Where as Non-genetic factors included in the study were location of herd,faming system and sequence of lactation.The average estimates of body weight of Graded murrah, Diara and Non-descript were found to be 508.972+3.36, 461.789+3.32 and 483.857+3.30 kg respectively. The three genetic groups of buffaloes differed significantly (p<0.05) among themselves with respect to their body weight.Farming system and lactation order had significant(p<0.01) influence on body weight. Body weight of the animals was the lowest at first parity and then increased significantly (p<0.05) in subsequent parities.
Key words :buffaloes ,Body weight,genetic and non genetic factors.


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Original Research

9.  Effect of polyherbal formulations on ruminal digestion in goat - P. B. Handekar, A. Y. Kolte, H. C. Mendhe, R. M. Puri, K. Ravikanth, S. Maini and D. S. Rekhe
Vet World. 2010; 3(5): 230-233

 

Rumen ecology plays vital role in the digestion, absorption and assimilation of ingesta in cattle. Digestive disorders in cattle directly affects the economics of dairy farm due to impact on production, hence the present study was planned to evaluate efficacy of herbal rumen ecology modulator AV/RMF/17 and Ruchamax on rumen liquor profile in goats. Total healthy twenty-one female goats were selected and divided randomly into three equal groups. Group (T1) was untreated control while, group T2 & T3 were supplemented with herbal Rumen modulator (AV/RMF/17) and Ruchamax (M/s Ayurvet Ltd. Baddi, HP) @ 1.5 kg/ tone of feed and 1 kg/ tone of feed for 45 days respectively. Physical, biochemical examination of rumen liquor and growth parameters were evaluated. Among physical properties colour, consistency and odour recorded on 0 day observed which was observed to be greenish brown with watery consistency while odour was slightly amonical, which changes to greenish brown with viscous consistency and aromatic odour in T2 and T3 group within 7th day post treatment. Biochemical examination of rumen liquor reveals significantly decreased pH, TTA, TVFA and lactic acid in control group which were significantly increased in T2 and T3 groups in post treatment period as compared to control. Growth parameters viz. body weight gain, feed consumption and FCR were also improved as compared to untreated control. From overall observation indicated that animal treated with AV/RMF/17 and Ruchamax therapies showed better results on physical, biochemical examination of rumen liquor and improvement in performance parameters indicates normalization of rumen microflora.
Key words: AV/RMF/17, Ruchamax, Goat, Rumen liquor, Microbial, FCR


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Case Report

10.  Clinical management of snake bite in a dog - B. R. Suchitra, M. C. Anilkumar and G. P. Kalmath
Vet World. 2010; 3(5): 234


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Case Report

11.  Mammary fibroadenoma in a rat and its surgical management - Mohd. Shafiuzama, M. Shiju Simon, B. C. Das, G. D. Rao, T. A. Kannan, and R. Suresh Kumar
Vet World. 2010; 3(5): 235

 

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Review Article

12.  Role of ß carotene / vitamin A in animal reproduction - Sudhir Kumar, A. K. Pandey, M. Mutha Rao and W. A. A. Razzaque
Vet World. 2010; 3(5): 236-237

 

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Review Article

13.  Use of assisted reproductive technologies for livestock development - Vikrama Chakravarthi. P and N. Sri Balaji
Vet World. 2010; 3(5): 238-240


Genetic improvement of farm animals is a prime concern over the years for researchers. Several reproductive technologies have been employed to achieve this. Assisted reproductive technologies like Artificial insemination, Superovulation, In vitro Fertilization, Embryo Transfer have been introduced to overcome reproductive problems, to increase the offspring from selected female’s and to reduce the generation intervals in farm animals. The progress achieved during the last few years in the assisted reproductive technologies field has been phenomenal. Artificial Insemination (AI) is the most effective method being used for the genetic improvement of animals. Reproductive capacity and efficiency has been improved tremendously since the introduction of artificial insemination. The development of cloning using various cells from the animal body has created opening of a fascinating scientific arena. These technologies have been propounded as saviors of indigenous livestock breeds. These alternative reproductive techniques are available not only for manipulation of reproductive processes but also proven to be powerful tools in curbing the spread of vertically transmitted diseases. The successful reproductive technologies such as AI and Embryo transfer need be applied on a large scale, emerging biotechnogies such as MOET, IVF and Cloning provides powerful tool for rapidly changing the animal populations, genetically. This advanced reproduction technologies will definitely play an important role in the future perspective and visions for efficient reproductive performance in livestock.
Key words: Oestrus Synchronization, AI, Cloning, MOET & IVF

 

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» doi: 10.5455/vetworld.2010.238-240


Review Article

14.  Rapid methods for detection of veterinary drug residues in meat - B P Shankar, B H Manjunatha, Prabhu, S Chandan, D Ranjith, V Shivakumar
Vet World. 2010; 3(5): 241-246


The use of substances having hormonal or thyreostatic action as well as b-agonists is banned in many countries. However, sometimes forbidden drugs may be added to feeds for illegal administration to farm animals for promoting increased muscle development or increased water retention and thus obtain an economical benefit. The result is a fraudulent overweight of meat but, what is worse, residues of these substances may remain in meat and may pose a real threat to the consumer either through exposure to the residues, transfer of antibiotic resistance or allergy risk. This has exerted a great concern among the meat consumers. The control of the absence of these forbidden substances in animal foods and feeds is regulated in the European Union by Directive 96/23/EC on measures to monitor certain substances and residues in live animals and animal products. Analytical methodology, including criteria for identification and confirmation, for the monitoring of compliance was also given in Decisions 93/256/EEC and 93/257/EEC. More recently, Decision 2002/657/EC provided rules for the analytical methods to be used in testing of official samples. New substances with anabolic properties are being detected year by year increasing the list of forbidden compounds to be tested. Furthermore, the extended practice consisting in the use of “cocktails” (mixtures of low amounts of several substances that exert a synergistic effect) to have a similar growth promotion, reduces the margin for an effective analytical detection. Thus, the evolution of the “black market” is making really difficult to have an effective analytical control of the residues of these substances in foods of animal origin. Control laboratories must face an increasing demand of analysis like the growing number of residues to be analysed in different types of samples, the strict guidelines for analytical methodologies according to the latest Directives, the increased costs of such new methodologies, the variety of residues to search per sample and the need to invest on powerful new instruments for identification and confirmatory purposes. Rapid and versatile screening methodologies make its control easier and reduce the number of non-compliant samples to be confirmed through tedious and costly confirmatory analytical methodologies. For instance, the multiresidue analysis can be performed better by using fast LC methods. Thus, the availability of new screening methodologies and the improvement of the existing ones will contribute to a better safety assurance of meat and other foods of animal origin.
Keywords: Drug Residue, Meat, Residues in food, Hormone.


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» doi: 10.5455/vetworld.2010.241-246


Review Article

15.  Toxoplasmosis : beware of cats !!! - Biswaranjan Maharana, Manjit Panigrahi, Rubina Kumari Baithalu, Subhashree Parida and Idrees Mehraj Allaie
Vet World. 2010; 3(5): 247-249


Anthropozoonotic parasite Toxoplasma gondii causes widespread human and animal diseases, mostly involving central nervous system. Human acquires toxoplasmosis from cats, from consuming raw or undercooked meat and from vertical transmission to the fetus through placenta from mother during pregnancy. Socio-epidemiological as well as unique environmental factors also plays a significant role in transmission of this infection. Preventive measures should be taken into account the importance of culture, tradition, and beliefs of people in various communities more than solving poverty and giving health education. Therefore the focus of this article is to create public awareness regarding sense of responsibility of looking after pets to prevent such an important zoonotic disease.
Keywords: Feline, Zoonosis, Vertical Transmission, Public Health.


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Review Article

16.  Managemental practices to control ascitis in a flock - Yancy Mary Issac, John Abraham, Sreeparvathy, Jini George and C. Balusami
Vet World. 2010; 3(5): 250-252


Ascitis is a metabolic disease of poultry in which excess amounts of fluid accumulates in the abdominal cavity. Ascitis is initiated by factors that elevate the blood pressure within arteries supplying the lungs. This increase in pulmonary arterial pressure (hypertension) triggers the accumulation of fluid in the abdominal cavity (ascitis). This condition, generally recognized in broilers is often lethal. If at all the bird survives, the carcass would most often be condemned. When birds are chronically exposed to low oxygen levels, it tend to lead to a high incidence of ascitis. Other predisposing factors associated with the syndrome are rapid tissue growth, low protein or high sodium chloride in the mash, ammonia, noxious gases and mycotoxin. Hepatitis, cardiovascular, pulmonary and renal diseases are also considered important in the etiology of ascitis syndrome. The birds affected show shrunken comb, loss of feathers in the abdominal region, subcutaneous odema, odema of head, cyanosis, pot bellied appearance, staggering gait and respiratory distress. It is better to prevent ascitis than going for treatment. Methods of feed restriction, Temperature, Air quality, Dust, Ammonia, Oxygen play a very useful role in control of ascitis.
Keywords: Management, Ascitis, Flock, Broiler, Abdomen, Renal Disease, Pulmonary Disease, Cardiovascular Disease.


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